Impact of feeding polyunsaturated fatty acids on cholesterol metabolism of dyslipidemic obese rats of WNIN/GR-Ob strain
Impact of feeding polyunsaturated fatty acids on cholesterol metabolism of dyslipidemic obese rats of WNIN/GR-Ob strain
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DOI：
10.1016/j.atherosclerosis.2008.08.021
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发表时间：
2009-05-01
期刊：
影响因子：
5.3
通讯作者：
Vajreswari, Ayyalasomayajula
中科院分区：
文献类型：
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作者：
Sheril, Alex;Jeyakumar, Shanmugam M.;Vajreswari, Ayyalasomayajula
众所周知，膳食脂肪酸在血脂异常的发生和预防中发挥着重要作用。在这项研究中，我们评估了喂养多不饱和脂肪酸 (PUFA) 4 个月对 WNIN/GR-Ob 品系遗传性肥胖突变大鼠胆固醇代谢各个方面的影响。根据表型，将瘦大鼠和肥胖大鼠分为两组，分别为A组和B组，并根据膳食脂肪的类型进一步细分。对照组大鼠（Al和BI）喂食4%花生油，然后用红花油代替；实验组为n-6 PUFA饮食（All和BII）或红花和大豆油的混合油，n-6和n-3 PUFA饮食（AIII和BIII）。据观察，与对照组（BI）相比，饲喂含有 n-6 PUFA 或 n-6 和 n-3 PUFA 组合的饮食会导致肥胖大鼠（BII 和 BIII）的血浆总胆固醇、HDL 胆固醇和甘油三酯水平显着升高。此外，肥胖大鼠的血浆 HDL 部分载脂蛋白 E (apo E) 升高，而 apo Al 水平保持不变。在 PUFA 喂养的肥胖大鼠（BII 和 BIII）中还观察到血浆中卵磷脂：胆固醇酰基转移酶 (LCAT) 活性和胆固醇酯 (CE) 水平增加，以及 B 型肝清道夫受体 1 型 (SR-B1) 表达增强。然而，在食用富含 PUFA 的饮食的肥胖大鼠中，肝脏 ATP 结合盒转运蛋白 A1 (ABCA1) 水平没有变化。有趣的是，尽管这些变化有利于从外周组织中有效去除胆固醇，但它的酯化作用和通过反向胆固醇转运（RCT）增强的清除率；在这些遗传性血脂异常肥胖大鼠中，血浆 HDL-C 仍然较高，从而表明涉及胆固醇稳态的尚不清楚的机制，需要研究。 (C) 2008 Elsevier Ireland Ltd. 保留所有权利。
Dietary fatty acids are known to play an important role in the development as well as prevention of dyslipidemia. In this study, we evaluated the impact of feeding polyunsaturated fatty acids (PUFAs) for a period of 4 months on various aspects of cholesterol metabolism in genetically obese mutant rats of WNIN/GR-Ob strain. Based on their phenotype, lean and obese rats were divided into two groups, A and B respectively, and further subdivided depending on the type of dietary fat. Control groups of rats (Al and BI), were fed on 4% groundnut oil, which was replaced by safflower oil; n-6 PUFA diet (All and BII) or oil blend of safflower and soybean oil, n-6 and n-3 PUFA diet (AIII and BIII) in the experimental groups. It was observed that feeding of diets with n-6 PUFA or a combination of n-6 and n-3 PUFAs resulted in marked elevation of plasma levels of total as well as HDL cholesterol and triglycerides in obese rats (BII and BIII), as compared to the control group (BI). Further, plasma HDL fraction of obese rats had elevated apolipoprotein E (apo E), while apo Al levels remained unaltered. Increased lecithin: cholesterol acyltransferase (LCAT) activity and cholesteryl ester (CE) levels in the plasma and enhanced expression of hepatic scavenger receptor class B type 1 (SR-B1) were also observed in PUFA-fed obese rats (BII and BIII). However, there was no change in hepatic ATP-binding cassette transporter protein A1 (ABCA1) levels in the obese rats fed on PUFA rich diets. Intriguingly, though these changes favor efficient removal of cholesterol from peripheral tissues, its esterification and enhanced clearance through reverse cholesterol transport (RCT); plasma HDL-C remained higher in these genetically dyslipidemic obese rats, thereby pointing at yet unknown mechanisms, involved in cholesterol homeostasis, which need to be studied. (C) 2008 Elsevier Ireland Ltd. All rights reserved.