Computer assisted semen analyzers in andrology research and veterinary practice
Computer assisted semen analyzers in andrology research and veterinary practice
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DOI：
10.1016/s0093-691x(01)00664-1
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发表时间：
2002-01-01
期刊：
影响因子：
2.8
通讯作者：
Onclin, K
中科院分区：
文献类型：
--
作者：
Verstegen, J;Iguer-Ouada, M;Onclin, K
精子活力和形态的评价是检查精子质量和建立精子质量与生育力之间相关性的重要参数。计算机辅助精子分析（CASA）可以客观评估不同的细胞特征：运动，速度和形态。在这篇综述中提出了与该技术相关的发展和问题，特别注意获得有用结果所绝对需要的偏差和标准化要求。虽然已经取得了一些有趣的结果，主要是在人类身上，但仍然存在许多问题，这些问题必须得到回答，以便在兽医学，临床生育环境，生理学和毒理学研究活动中进一步发展这项技术。主要问题涉及设备和程序的标准化和优化。使用不同的精子分类方法，不同的CASA仪器都表现出高水平的精确度和可靠性。它们的可用性为我们提供了一个很好的工具，可以客观地比较精子活力和形态，并提高我们操纵精子的知识和能力。(C)2001年，Elsevier Science Inc.
The evaluation of sperm cell motility and morphology is an essential parameter in the examination of sperm quality and in the establishment of correlations between sperm quality and fertility. Computer-assisted sperm analysis (CASA) allows an objective assessment of different cell characteristics: motion, velocity, and morphology. The development and problems related to this technology are raised in this review, paying particular attention to the biases and standardization requirements absolutely needed to obtain useful results. Although some interesting results, mainly in humans, have already been obtained, many questions remain, which have to be answered to allow for further development of this technology in veterinary medicine, clinical fertility settings, physiological, and toxicology research activities.The main problem is related to the standardization and optimization of the equipment and procedures. The different CASA instruments have ail demonstrated high levels of precision and reliability using different sperm classification methodology. Their availability gives us a great tool to objectively compare sperm motility and morphology and to improve our knowledge and ability to manipulate spermatozoa. (C) 2001 by Elsevier Science Inc.