Live-cell nanoscopy with spontaneous blinking of conventional green fluorescent proteins
Live-cell nanoscopy with spontaneous blinking of conventional green fluorescent proteins
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DOI：
10.1016/j.bbrc.2019.11.163
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发表时间：
2020-02-19
影响因子：
3.1
通讯作者：
Mishin, Alexander S.
中科院分区：
文献类型：
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作者：
Gavrikov, Alexey S.;Baranov, Mikhail S.;Mishin, Alexander S.
具有自发闪烁荧光标记的单分子定位显微镜为活细胞应用提供了简化的成像设置。然而，据报道，只有少数荧光蛋白具有强烈的闪烁。在这里，我们报告的比较自发闪烁的三个明亮的绿色荧光蛋白，mAvicFP 1，AausFP 1，和mNeon绿色。mAvicFP1在广泛的相机曝光时间和照明强度方面优于该列表中的其他荧光蛋白。我们建立了活细胞纳米显微镜和单粒子跟踪mAvicFP1的成像条件。(C)2019爱思唯尔公司All rights reserved.
Single-molecule localization microscopy with spontaneously blinking fluorescent tags holds a promise of simplified imaging setup for live-cell application. However, robust blinking has been reported for just a few fluorescent proteins. Here we report on a comparison of spontaneous blinking for three bright green fluorescent proteins, mAvicFP1, AausFP1, and mNeonGreen. mAvicFP1 outperforms other fluorescent proteins in this list in a wide range of camera exposure times and illumination intensities. We establish imaging conditions for live-cell nanoscopy and single-particle tracking with mAvicFP1. (C) 2019 Elsevier Inc. All rights reserved.