The amygdala mediates the anxiolytic-like effect of the neurosteroid allopregnanolone in rat
The amygdala mediates the anxiolytic-like effect of the neurosteroid allopregnanolone in rat
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DOI：
10.1016/s0166-4328(99)00101-1
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发表时间：
1999-12-01
影响因子：
2.7
通讯作者：
Britton, KT
中科院分区：
文献类型：
--
作者：
Akwa, Y;Purdy, RH;Britton, KT
神经甾体别孕烯醇酮（3α -羟基- 5α -孕甾- 20 -酮）具有明显的类抗焦虑作用。其他神经甾体，即硫酸孕烯醇酮（PREG - S）和硫酸脱氢表雄酮（DHEA - S）对焦虑相关行为的影响有所不同。在本研究中，对杏仁核（恐惧和焦虑机制中的一个关键结构）的作用进行了研究，以探讨其作为神经甾体对大鼠类焦虑行为影响的潜在神经基质。将双侧套管植入大鼠杏仁核中央核（CeA）并注入神经甾体，然后在两种焦虑动物模型中对动物进行测试。在冲突测试中，别孕烯醇酮（每侧8微克）使受惩罚抑制的反应显著增加。在高架十字迷宫中，与注入溶媒的对照组相比，别孕烯醇酮（每侧8微克）使大鼠在开放臂中停留的时间和进入开放臂的次数显著增加。将PREG - S（每侧0.001 - 8微克）和DHEA - S（每侧2 - 8微克）注入CeA或侧脑室（1 - 20微克）时，在冲突测试中未观察到受惩罚和未受惩罚反应有显著变化。结果表明，PREG - S和DHEA - S在操作性冲突测试中无活性，但提示杏仁核中央核是别孕烯醇酮类抗焦虑作用机制所涉及的一个关键区域。（C）1999年，爱思唯尔科学出版社。版权所有。
The neurosteroid allopregnanolone (3 alpha-hydroxy-5 alpha-pregnan-20-one) possesses clear anxiolytic-like effects. Other neurosteroids namely pregnenolone sulfate (PREG-S) and dehydroepiandrosterone sulfate (DH EA-S) influence anxiety-related behavior differently. In the present study, the implication of the amygdala, a key structure in mechanisms of fear and anxiety, was investigated as a potential neural substrate for the effects of neurosteroids on anxiety-like behavior in rat. Animals implanted with bilateral cannulae aimed at the central nucleus of the amygdala (CeA) and infused with neurosteroids, were tested in two animal models of anxiety. Allopregnanolone (8 mu g/side) produced a significant increase in responding suppressed by punishment in the conflict test. In the elevated plus maze, allopregnanolone (8 mu g/side) induced a significant increase in the time spent and the number of entries in open arms compared with the vehicle-infused controls. No significant changes in punished and unpunished responding of the conflict test were observed with PREG-S (0.001-8 mu g/side) and DHEA-S (2-8 mu g/side) administered into the CeA or into the lateral ventricle (1-20 mu g). The results reveal the lack of activity of PREG-S and DHEA-S in the operant conflict rest, but suggest that the central nucleus of the amygdala is a key region involved in the mechanisms underlying the anxiolytic-like action of allopregnanolone. (C) 1999 Elsevier Science B.V. All rights reserved.