Isolation and characterization of Chlamydomonas temperature-sensitive mutants affecting gametic differentiation under nitrogen-starved conditions.
Isolation and characterization of Chlamydomonas temperature-sensitive mutants affecting gametic differentiation under nitrogen-starved conditions.
复制标题
衣藻温度敏感突变体在氮饥饿条件下影响配子分化的分离和表征。
DOI：
10.1007/bf00326284
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发表时间：
1991
期刊：
影响因子：
2.5
通讯作者：
Matsuda,Y
中科院分区：
文献类型：
--
作者：
Saito,T;Matsuda,Y
在氮饥饿条件下，莱茵衣藻（Chlamydiumreinhardtii）的两个条件突变体dif-1和dif-2能影响配子分化。这些突变体细胞在限制性温度（35°C）下在- N培养基中保持“营养”，如通过细胞匀浆的可溶性部分中的细胞体凝集素和细胞壁裂解酶活性的测定所定义的。此外，突变体在限制温度下不能形成交配结构，但在允许温度（25°C）下可以形成交配结构。温度变化实验表明，在25°C下分化为配子的突变细胞在转移到35°C后在N饥饿条件下去分化为“营养”细胞，但在25°C下再次分化为配子。遗传分析表明，dif-1和dif-2基因是隐性的，彼此不连锁，也不与交配型位点连锁; dif-1表型与限制性温度下在+N和-N培养基中表达的条件鞭毛表型共分离。
Two conditional mutants ofChlamydomonas reinhardtii, dif-1anddif-2, affecting gametic differentiation under conditions of nitrogen (N)-starvation, have been isolated. These mutant cells remain “vegetative” at the restrictive temperature (35°C) in — N medium, as defined by assays of cell body-agglutinin and cell wall lytic enzyme activities in the soluble fractions of cell homogenates. Moreover, the mutants fail to form mating structures at the restrictive temperature, but do so at the permissive temperature (25°C). Temperature-shift experiments show that mutant cells which have differentiated into gametes at 25°C dedifferentiate into “vegetative” cells under N-starvation conditions after transfer to 35°C, but differentiate again into gametes at 25°C. Genetic analyses indicate that thedif-1anddif-2genes are recessive and unlinked to each other or to the matingtype locus; thedif-1phenotype cosegregates with a conditional flagellales phenotype expressed in both +N and-N medium at the restrictive temperature.