Synergistic Interactions of Pseudomonas aeruginosa and Staphylococcus aureus in an In Vitro Wound Model
Synergistic Interactions of Pseudomonas aeruginosa and Staphylococcus aureus in an In Vitro Wound Model
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DOI：
10.1128/iai.02198-14
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发表时间：
2014-11-01
影响因子：
3.1
通讯作者：
Rumbaugh, Kendra P.
中科院分区：
文献类型：
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作者：
DeLeon, Stephanie;Clinton, Allie;Rumbaugh, Kendra P.
在多微生物感染的个体中，微生物经常表现出协同作用，可以增强其定植，毒力或持久性。最常见的多微生物感染类型之一发生在慢性伤口，其中铜绿假单胞菌和金黄色葡萄球菌是两种最常见的原因。虽然它们是伤口感染中最常见的相关微生物物种，但对它们的种间关系知之甚少。有证据表明铜绿假单胞菌- s。金黄色葡萄球菌共感染比单种感染的毒性更强；然而，在体外同时培养这两种病原体的困难阻碍了揭示相关机制的尝试。在这里，我们描述了一个简单的和临床相关的体外伤口模型，支持铜绿假单胞菌和金黄色葡萄球菌的共同生长。我们观察到，铜绿假单胞菌和金黄色葡萄球菌在浮游共培养中共同培养时，对抗生素治疗的生存能力增强，在伤口模型中共同培养时，抗生素耐受性进一步增强。我们将这种增强的耐受性归因于“宿主衍生”和“细菌衍生”的基质成分。综上所述，我们的数据表明铜绿假单胞菌和金黄色葡萄球菌可能通过共同感染伤口而相互受益，并且宿主来源的基质可能在保护细菌免受某些抗生素侵害方面发挥与细菌来源基质同样重要的作用。
In individuals with polymicrobial infections, microbes often display synergistic interactions that can enhance their colonization, virulence, or persistence. One of the most prevalent types of polymicrobial infection occurs in chronic wounds, where Pseudomonas aeruginosa and Staphylococcus aureus are the two most common causes. Although they are the most commonly associated microbial species in wound infections, very little is known about their interspecies relationship. Evidence suggests that P. aeruginosa-S. aureus coinfections are more virulent than monoculture infection with either species; however, difficulties in growing these two pathogens together in vitro have hampered attempts to uncover the mechanisms involved. Here we describe a simple and clinically relevant in vitro wound model that supported concomitant growth of P. aeruginosa and S. aureus. We observed that the ability of P. aeruginosa and S. aureus to survive antibiotic treatment increased when they were grown together in planktonic cocultures and that antibiotic tolerance was further enhanced when they were grown together in the wound model. We attributed this enhanced tolerance to both the "host-derived" and "bacterium-derived" matrix components. Taken together, our data indicate that P. aeruginosa and S. aureus may benefit each other by coinfecting wounds and that the host-derived matrix may serve as important a role as the bacterium-derived matrix in protecting bacteria from some antibiotics.