CARRIER RATE OF STREPTOCOCCUS-SUIS CAPSULAR TYPE-2 IN PALATINE TONSILS OF SLAUGHTERED PIGS
CARRIER RATE OF STREPTOCOCCUS-SUIS CAPSULAR TYPE-2 IN PALATINE TONSILS OF SLAUGHTERED PIGS
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DOI：
10.1128/jcm.20.5.945-947.1984
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发表时间：
1984-01-01
影响因子：
9.4
通讯作者：
ZANEN, HC
中科院分区：
文献类型：
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作者：
ARENDS, JP;HARTWIG, N;ZANEN, HC
对143头4 ~ 6月龄屠宰猪的腭扁桃体进行了猪链球菌2型感染调查。切片（50 .亩）m)冷冻扁桃体在含有抗猪链球菌2型抗体的选择性琼脂培养基上培养，这种细菌的菌落显示出免疫沉淀的晕。143头猪中有45头（32%）在1个平面扁桃体切片时发现猪链球菌2型。在55个扁桃体上按bbb1平面切片时，这一比例增加到50%。对前45株显示免疫沉淀环的菌株进行了研究，发现其生化性质与参考菌株735 （de Moor）和从脑膜炎患者中分离的23株相同。37度孵育24小时。在固定后用苏木精和伊红染色，可以证明猪链球菌2型局限于隐窝腔内。直接固定（不孵育）的切片，用兔抗s间接免疫过氧化物酶法染色，结果同样如此。猪2型血清。
Palatine tonsils of 143 slaughtered pigs 4-6 mo. old were investigated for the presence of S. suis type 2. Slices (50 .mu.m) of frozen tonsils were cultured on a selective agar medium containing antibodies against S. suis type 2 in which colonies of this bacterium showed a halo of immunoprecipitation. When tonsils were sectioned in 1 plane S. suis type 2 was found in 45 of 143 pigs (32%). This percentage increased to 50% when tonsils were sectioned in > 1 plane, which was done on 55 tonsils. The first 45 strains showing a ring of immunoprecipitation were studied and found to be biochemically identical to the reference strain 735 (de Moor) and to 23 isolates from human patients with meningitis. In slices incubated for 24 h at 37.degree. C on selective agar plates and stained with hematoxylin and eosin after fixation, it could be demonstrated that S. suis type 2 was confined to the crypt lumen. The same was true in sections fixed directly (without incubation) that were stained by an indirect immunoperoxidase method with a rabbit anti-S. suis type 2 serum.