Hormonal control of arylhydrocarbon receptor (AhR) expression in the preimplantation rabbit uterus
Hormonal control of arylhydrocarbon receptor (AhR) expression in the preimplantation rabbit uterus
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DOI：
10.1007/s004290100209
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发表时间：
2001-09-01
期刊：
影响因子：
--
通讯作者：
Fischer, B
中科院分区：
文献类型：
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作者：
Hasan, A;Fischer, B
我们发现芳基烃受体（AhR）以独特的模式定位在植入前的兔子宫中。采用免疫组织化学方法，分别对成熟和未成熟兔在植入前不同妊娠阶段（非妊娠/动情、妊娠和假妊娠）以及雌二醇和黄体酮治疗后的子宫进行研究。怀孕和假怀孕兔子的染色模式相似，表明表达的主要母体控制。 AhR 定位的明显变化发生在 (1) 与怀孕兔子相比的未怀孕兔子和 (2) 在预期着床时间之前不久的子宫内膜中。与非妊娠子宫相比，妊娠子宫内膜上皮细胞的AhR不再局限于细胞核顶端的小细胞质区域，而是存在于整个细胞质和细胞核中。在植入前的第 6 天，基质细胞和子宫腺体上皮（但不再是腔上皮细胞）呈免疫阳性。雌二醇处理的未成熟子宫在管腔上皮和一些子宫腺体中显示出强烈的表达，通常具有核染色，而雌二醇引发和黄体酮处理的标本具有与成熟植入前子宫相似的染色模式。目前的结果表明，在植入前期间，AhR 在子宫内膜中具有独特的定位模式。表达模式的阶段特异性变化表明 AhR 表达受到母体类固醇激素的控制。
We show that the arylhydrocarbon receptor (AhR) is localised in a distinct pattern in the preimplantation rabbit uterus. Employing immunohistochemistry, uteri from mature and immature rabbits were studied in different pregnancy stages during the preimplantation period (non-pregnant/estrous, pregnant and pseudopregnant) and following treatment with estradiol and progesterone, respectively. The staining patterns in pregnant and pseudopregnant rabbits were similar, indicating a primary maternal control of expression. A distinct change in AhR localization occurred in the endometrium of (1) non-pregnant compared with pregnant rabbits and (2) shortly prior to the anticipated time of implantation. Compared with non-pregnant uteri, the AhR was no longer restricted to a small cytoplasmic area apical to the nucleus but was found in the entire cytoplasm and in the nuclei of the endometrial epithelial cells in pregnant uteri. Prior to implantation, at day 6, stromal cells and the epithelium of the uterine glands, but no longer the luminal epithelial cells, were immunopositive. The estradiol-treated immature uteri showed a strong expression in the luminal epithelium and in some uterine glands, often with nuclear staining, while the estradiol-primed and progesterone-treated specimens had a similar staining pattern as mature preimplantation uteri. Present results demonstrate a distinct localization pattern of the AhR in the endometrium during the preimplantation period. Stage-specific changes in the expression pattern indicate control of AhR expression by maternal steroid hormones.