Proteomic study of retinal proteins associated with transcorneal electric stimulation in rats.
Proteomic study of retinal proteins associated with transcorneal electric stimulation in rats.
复制标题
DOI：
10.1155/2015/492050
复制
发表时间：
2015
影响因子：
1.9
通讯作者：
Kiuchi Y
中科院分区：
文献类型：
--
作者：
Kanamoto T;Souchelnytskyi N;Kurimoto T;Ikeda Y;Sakaue H;Munemasa Y;Kiuchi Y
背景。通过鉴定大鼠视网膜中经角膜电刺激（TES）上调和下调的蛋白，探讨经角膜电刺激（TES）对视网膜的影响。方法。成年Wistar大鼠在不同电流下对左眼进行TES，而右眼不做任何处理，作为对照。经TES治疗后，将眼球去核并分离视网膜。视网膜进行蛋白质组学分析。结果。蛋白质组学结果显示，TES上调了25个蛋白。鉴定的蛋白包括细胞信号蛋白、与神经元传递相关的蛋白、代谢蛋白、免疫因子和结构蛋白。结论。TES诱导视网膜内各种功能蛋白表达的变化。
Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina.