Highly sensitive disposable nucleic acid biosensors for direct bioelectronic detection in raw biological samples.
Highly sensitive disposable nucleic acid biosensors for direct bioelectronic detection in raw biological samples.
复制标题
DOI：
10.1016/j.talanta.2011.06.012
复制
发表时间：
2011-09-15
期刊：
影响因子：
6.1
通讯作者：
Wang, Joseph
中科院分区：
文献类型：
--
作者：
Kuralay, Filiz;Campuzano, Susana;Haake, David A.;Wang, Joseph
开发快速、低成本和可靠的诊断方法对于许多疾病的识别和治疗至关重要。丝网印刷的金电极（Au/SPE），涂有一个三元单层界面，涉及己二硫醇（HDT），一个特定的硫醇化捕获探针（SHCP），和6-巯基-1己醇（MCH）（SHCP/HDT/MCH），在这里显示提供直接和灵敏的检测核酸杂交事件在未经处理的原始生物样品（血清，尿液和粗细菌裂解液解决方案）。三元单分子膜的组成进行了修改和定制的表面的Au/SPE。所得的SHCP/HDT/MCH单层已被证明是非常有用的，用于增强基于丝网印刷电极的一次性核酸传感器的性能。与常见的SHCP/MCH二元界面相比，新的三元自组装单层（SAM）导致1 nM靶DNA的信噪比（S/N）提高了10倍。SHCP/HDT/MCH修饰的Au/SPE允许在未稀释/未处理的血清和尿液样品中分别直接定量低至25 pM（0.25 fmol）和100 pM（1 fmol）的靶DNA，以及16 S rRNA大肠杆菌（E.大肠杆菌），对应于原始细胞裂解物样品中的3000 CFU μL−1。新的SAM涂层丝网印刷电极在暴露于原始人类血清和尿液样品24小时后也显示出良好的防污性能，为广泛的分散式基因检测提供了具有成本效益的核酸传感器。
The development of rapid, low-cost and reliable diagnostic methods is crucial for the identification and treatment of many diseases. Screen-printed gold electrodes (Au/SPEs), coated with a ternary monolayer interface, involving hexanedithiol (HDT), a specific thiolated capture probe (SHCP), and 6-mercapto-1 hexanol (MCH) (SHCP/HDT/MCH) are shown here to offer direct and sensitive detection of nucleic acid hybridization events in untreated raw biological samples (serum, urine and crude bacterial lysate solutions). The composition of the ternary monolayer was modified and tailored to the surface of the Au/SPE. The resulting SHCP/HDT/MCH monolayer has demonstrated to be extremely useful for enhancing the performance of disposable nucleic acid sensors based on screen-printed electrodes. Compared to common SHCP/MCH binary interfaces, the new ternary self-assembled monolayer (SAM) resulted in a 10-fold improvement in the signal (S)-to-noise (N) ratio (S/N) for 1 nM target DNA. The SHCP/HDT/MCH-modified Au/SPEs allowed the direct quantification of the target DNA down to 25 pM (0.25 fmol) and 100 pM (1 fmol) in undiluted/untreated serum and urine samples, respectively, and of 16S rRNA Escherichia coli (E. coli) corresponding to 3000 CFU μL−1 in raw cell lysate samples. The new SAM-coated screen-printed electrodes also displayed favorable non-fouling properties after a 24 h exposure to raw human serum and urine samples, offering great promise as cost-effective nucleic acid sensors for a wide range of decentralized genetic tests.
登录
查看更多内容
影响因子：
12.6
作者：
Lucarelli, F;Marrazza, G;Mascini, M
通讯作者：
Mascini, M
影响因子：
9.4
作者：
Liao, JC;Mastali, M;Haake, DA
通讯作者：
Haake, DA
影响因子：
4.5
作者：
Campuzano, S;Gálvez, R;Pingarrón, JM
通讯作者：
Pingarrón, JM
影响因子：
3.9
作者：
Boozer, C;Chen, SF;Jiang, SY
通讯作者：
Jiang, SY
影响因子：
4.5
作者：
Campuzano, S;Pedrero, M;Pingarrón, JM
通讯作者：
Pingarrón, JM