Structural characterization of EasH (Aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.
Structural characterization of EasH (Aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.
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DOI：
10.1039/c6cc08438a
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发表时间：
2016-12-06
期刊：
影响因子：
--
通讯作者：
O'Connor SE
中科院分区：
文献类型：
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作者：
Jakubczyk D;Caputi L;Stevenson CE;Lawson DM;O'Connor SE
Aj_EasH是一种非血红素铁和α-酮戊二酸依赖性氧化酶，负责真菌麦角生物碱环棒麦角碱生物合成中不寻常的环丙基环形成。Aj_EasH是一种非血红素铁和α-酮戊二酸依赖性氧化酶，负责真菌麦角生物碱环棒麦角碱生物合成中不寻常的环丙基环形成。本文报道的Aj_EasH的三维结构（2.2 μ m分辨率）提供了对这种不寻常的复杂反应机制的深入了解。
Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.