Nuclear movement during myotube formation is microtubule and dynein dependent and is regulated by Cdc42, Par6 and Par3
Nuclear movement during myotube formation is microtubule and dynein dependent and is regulated by Cdc42, Par6 and Par3
复制标题
DOI：
10.1038/embor.2012.89
复制
发表时间：
2012-08-01
期刊：
影响因子：
7.7
通讯作者：
Gomes, Edgar R.
中科院分区：
文献类型：
--
作者：
Cadot, Bruno;Gache, Vincent;Gomes, Edgar R.
细胞主动地将其核定位在细胞质内。一个突出的例子是在骨骼肌发生过程中观察到的。分化的成肌细胞融合形成多核肌管，细胞核位于合胞体的中心，其机制未知。在这里，我们描述了成肌细胞的细胞核融合后迅速向中央肌管细胞核移动。这种运动由微管和动力蛋白/动力蛋白复合物驱动，需要Cdc 42、Par 6和Par 3。我们发现，Par 6 b和dynactin积累在核膜的分化成肌细胞和肌管，这种积累是依赖于Par 6和Par 3蛋白，但不对微管。这些结果表明，融合后的核运动是由微管驱动的机制，微管从一个细胞核发出，被锚定到另一个细胞核的核膜的动力蛋白/动力肌动蛋白复合物拉动。
Cells actively position their nucleus within the cytoplasm. One striking example is observed during skeletal myogenesis. Differentiated myoblasts fuse to form a multinucleated myotube with nuclei positioned in the centre of the syncytium by an unknown mechanism. Here, we describe that the nucleus of a myoblast moves rapidly after fusion towards the central myotube nuclei. This movement is driven by microtubules and dynein/dynactin complex, and requires Cdc42, Par6 and Par3. We found that Par6b and dynactin accumulate at the nuclear envelope of differentiated myoblasts and myotubes, and this accumulation is dependent on Par6 and Par3 proteins but not on microtubules. These results suggest a mechanism where nuclear movement after fusion is driven by microtubules that emanate from one nucleus that are pulled by dynein/dynactin complex anchored to the nuclear envelope of another nucleus.