Xylaria hypoxylon Lectin as Adjuvant Elicited Tfh Cell Responses
Xylaria hypoxylon Lectin as Adjuvant Elicited Tfh Cell Responses
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Xylaria hyperxylon 凝集素作为佐剂引发 Tfh 细胞反应
DOI：
10.1111/sji.12349
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发表时间：
2015-11-01
影响因子：
3.7
通讯作者：
Kang, Y.
中科院分区：
文献类型：
--
作者：
Kang, J.;Zuo, Y.;Kang, Y.
由口蹄疫病毒（FMD virus，FMDV）引起的口蹄疫（Foot-and-mouth disease，FMD）是一种严重危害人类健康的疾病。为牲畜接种疫苗对抗这种高度传染性的病毒性疾病是至关重要的，灭活口蹄疫疫苗在控制感染方面是有效的。然而，累积的数据显示灭活疫苗产生弱的免疫应答，并且油制剂导致不期望的副作用。蘑菇凝集素最近被证明在掺入DNA疫苗中时显示佐剂作用。为增强口蹄疫病毒（FMDV）抗原（146 S）的细胞免疫应答，将146 S与木糖炭角菌凝集素（XHL）联合免疫C57 BL/6小鼠。油制剂（146 S/油）作为对照组。146 S/XHL免疫小鼠可产生较强的体液免疫应答，146 S/Oil组也可产生较高的146 S抗原特异性IgG水平。有趣的是，XHL与灭活口蹄疫疫苗结合在免疫小鼠中激活了强烈的Th 1和Tc 1细胞应答，尤其是Tfh细胞应答。XHL通过上调免疫小鼠中主要组织相容性复合物II（MHCII）分子和共刺激分子CD 40和CD 86的表达来刺激树突状细胞成熟。未检测到XHL特异性IgG或炎性因子，表明XHL作为佐剂的安全性。综上所述，这些结果表明XHL在诱导细胞免疫应答方面的有效性，因此证实其作为灭活口蹄疫疫苗佐剂的适用性。
Foot-and-mouth disease (FMD) caused by FMD virus (FMDV) is a major health and economic problem in the farming industry. Vaccination of livestock against this highly infectious viral disease is crucial, and inactivated FMD vaccine has been effective at controlling infection. However, accumulated data show that the inactivated vaccine generates weak immune responses and that the oil formulation results in undesirable side effects. Mushroom lectins have recently been shown to display adjuvant effects when incorporated into DNA vaccines. In this study, to enhance the cellular immune response of FMDV antigen (146S), C57BL/6 mice were immunized with 146S combined with Xylaria hypoxylon lectin (XHL). The oil formulation (146S/Oil) was served as control group. Strong humoral immune responses were elicited in mice immunized with 146S/XHL as shown by high 146S antigen-specific IgG levels, and also in 146S/Oil group. Interestingly, XHL in conjunction with inactivated FMD vaccine activated strong Th1 and Tc1 cell responses, especially Tfh cell responses, in immunized mice. XHL stimulated dendritic cell maturation by upregulating expression of major histocompatibility complex II (MHCII) molecules and co-stimulatory molecules CD40 and CD86 in immunized mice. No XHL-specific IgG or inflammatory factors were detected indicating the safety of XHL as an adjuvant. Taken together, these results suggest the effectiveness of XHL at inducing cellular immune responses and therefore confirm its suitability as an adjuvant for inactivated FMD vaccine.