Cytokine secretion is impaired in women with diabetes mellitus
Cytokine secretion is impaired in women with diabetes mellitus
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DOI：
10.1046/j.1365-2362.2000.00745.x
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发表时间：
2000-11-01
影响因子：
5.5
通讯作者：
Hoepelman, AIM
中科院分区：
文献类型：
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作者：
Geerlings, SE;Brouwer, EC;Hoepelman, AIM
背景由于糖尿病(DM)妇女无症状性菌尿(ASB)的发病率增加，并且已知在感染人类免疫缺陷病毒(HIV)的妇女中，生殖道感染的发病率增加与细胞因子分泌受损之间存在相关性，我们研究了糖尿病妇女的尿细胞因子排泄，并将其与非糖尿病对照组进行比较。结果尿液中白细胞介素8(IL-8)和白细胞介素6(IL-6)浓度明显降低(P=0.1和P<；分别为0.001)，糖尿病女性的发病率高于非糖尿病对照组。尿白细胞计数降低与尿IL-8和IL-6浓度降低相关(P<；0.05)。与非糖尿病组相比，1型糖尿病组全血(P<；0.04)和单核细胞(P=0.03)中肿瘤坏死因子-α(TNF-α)和IL-6水平降低，而白介素10(IL-10)水平无明显差异。结论糖尿病合并ASB患者尿IL-6水平低于非糖尿病菌尿对照组。此外，1型糖尿病患者的单核细胞在脂多糖刺激后分泌的促炎细胞因子低于非糖尿病患者的单核细胞。这不是由于抗炎细胞因子IL-10的抑制作用。这可能会对宿主防御、内皮细胞功能和动脉粥样硬化形成产生重要影响。
Background As women with diabetes mellitus (DM) have an increased prevalence of asymptomatic bacteriuria (ASB) and it is known that a correlation exists between the increased prevalence of genitourinary tract infection and impaired cytokine production in women infected with Human Immunodeficiency Virus (HIV), we studied urinary cytokine excretion in diabetic women and compared it with that of nondiabetic controls.Materials and methods To evaluate the cytokine secretion capacity of women with DM, both whole blood and isolated monocytes of women with and without DM were stimulated in vitro with lipopolysaccharide (LPS).Results Lower urinary interleukin-8 (IL-8) and interleukin-6 (IL-6) concentrations (P = 0.1 and P < 0.001, respectively) were found in diabetic women than in nondiabetic controls. A lower urinary leukocyte cell count correlated with lower urinary IL-8 and IL-6 concentrations (P < 0.05). Lower tumour necrosis factor-alpha (TNF-a) and IL-6, but comparable interleukin-10 (IL-10) concentrations were found in whole blood (P < 0.04) and isolated monocytes (P = 0.03) of women with DM type 1 compared to women without DM.Conclusions Diabetic women with ASB have lower urinary IL-6 concentrations than nondiabetic bacteriuric controls. In addition, monocytes of women with DM type 1 secrete lower pro-inflammatory cytokines after stimulation with LPS than monocytes of women without DM. This is not due to an inhibitory effect of the anti-inflammatory cytokine IL-10. This can have important consequences for both host defense, endothelial cell functioning and atherogenesis.