Runx3 expression during mouse tongue and palate development
Runx3 expression during mouse tongue and palate development
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DOI：
10.1002/ar.a.20339
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发表时间：
2006-07-01
期刊：
影响因子：
--
通讯作者：
Ito, Yoshiaki
中科院分区：
文献类型：
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作者：
Yamamoto, Hiromitsu;Ito, Kosei;Ito, Yoshiaki
哺乳动物口腔粘膜发育的分子机制研究揭示了转化生长因子-β(TGF-β)和骨形态蛋白(BMP)信号通路的重要参与。在这里，我们研究了转化生长因子-β和骨形态发生蛋白的下游靶标Runx3在口腔粘膜中的表达。RUNX3是一种矮小相关转录因子，在哺乳动物胃上皮细胞中作为胃肿瘤抑制因子和生长分化调节因子发挥作用。线虫中RUNX家族的另一成员Run参与了功能皮下组织和肠道的发育。在这篇报道中，我们用逆转录聚合酶链式反应、免疫组织化学和原位杂交检测了Runx3的表达，发现Runx3在小鼠胚胎12.5天到16.5天的舌部和腭部上皮中都有表达。讨论了Runx3与转化生长因子-β/骨形态发生蛋白信号在舌和腭部发育中的功能关系。
Studies of molecular mechanisms underlying the development of the mammalian oral mucosa have revealed a major involvement of transforming growth factor beta (TGF-beta) and bone morphologic protein (BMP) signaling pathways. Here, we examined the expression of a downstream target of TGF-beta and BMPs, Runx3, in oral mucosa. Runx3 is a runt-related transcription factor that acts as a gastric tumor suppressor and regulator of growth and differentiation in mammalian gastric epithelial cells. Another member of the Runx family in C. elegans, run, is invoflved in the development of a functional hypodermis and gut. In this report, we examined Runx3 expression using reverse transcription-polymerase chain reaction, immnunohistochemistry, and in situ hybridization and found that Runx3 is expressed in the tongue and palate epithelium of mouse embryos from embryonic day 12.5 to 16.5. The functional relationship between Runx3 and TGF-beta/BMPs signaling in tongue and palate development is discussed.