HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR LABETALOL IN HUMAN-PLASMA USING A PRP-1 COLUMN AND FLUOROMETRIC DETECTION
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR LABETALOL IN HUMAN-PLASMA USING A PRP-1 COLUMN AND FLUOROMETRIC DETECTION
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DOI：
10.1016/s0378-4347(00)84725-8
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
PATRICK, J
中科院分区：
文献类型：
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作者：
ALTON, KB;LEITZ, F;PATRICK, J
A high-performance liquid chromatographic assay for the determination of labetalol, a novel antihypertensive agent, in human plasma was developed. Reversed-phase separation of labetalol and the internal standard was accomplished on a 150 .times. 4.1 mm column commercially packed with a spherical (8-12 .mu.m particle size) macroporous co-polymer (RPR-1). Unlike silica-based columns, the unique properties of PRP-1 permit operation at pH extremes. Based on this advantage, a mobile phase which was sufficiently basic (pH 9.5) to optimize the fluorescent yield of analyte and provide the necessary specificity was selected. Detector response (peak area ratio) was linear from 4-500 ng/nl. Following a simple extraction procedure, samples were automatically injected and analyzed using microprocessor-controlled equipment. No interferences were observed in the extracts obtained from drug-free plasma which were processed under the conditions described for unchanged drug. The limit of quantitation using 0.5 ml of plasma was validated to 4 ng/ml. The inter-assay precision (coefficient of variation) was < 4.6% at all concentrations evaluated from 4-300 ng/ml. This method was suitable for the routine quantitation of labetalol or its RR isomer (dilevalol) in plasma (0-24 h) following the administration of therapeutically effective doses to man.
