Visualization of Immune Cell Reconstitution by Bioluminescent Imaging.
Visualization of Immune Cell Reconstitution by Bioluminescent Imaging.
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通过生物发光成像观察免疫细胞重建。
DOI：
10.1007/978-1-4939-7860-1_10
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Dubey,Purnima
中科院分区：
文献类型：
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作者：
Dubey,Purnima
基于报告基因的分子成像技术是检测体内不同细胞群体运动和功能的有力手段。用分子成像报告基因标记的免疫系统的重建允许在实验系统中追踪对病原体和癌症的初级免疫应答。本章描述了分离骨髓干/祖细胞的方法，用成像报告基因标记它们，并通过生物发光成像跟踪外周免疫系统的重建。
Reporter gene-based molecular imaging is a powerful means to detect the movement and function of diverse cell populations in vivo. Reconstitution of an immune system marked with molecular imaging reporter genes permits tracking of primary immune responses to pathogens and cancer in experimental systems. This chapter describes the methods to isolate bone marrow stem/progenitors, transduce them with imaging reporter genes, and track the reconstitution of the peripheral immune system by bioluminescent imaging.