Effect of acupotomy on nitric oxide synthase and beta-endorphin in third lumbar vertebrae transverse process syndrome model rats
Effect of acupotomy on nitric oxide synthase and beta-endorphin in third lumbar vertebrae transverse process syndrome model rats
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DOI：
10.1016/s0254-6272(14)60078-9
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发表时间：
2014-04-15
影响因子：
2.6
通讯作者：
Wu, Haixia
中科院分区：
文献类型：
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作者：
Guo, Changqing;Liu, Naigang;Wu, Haixia
目的：为探讨针刀治疗第3腰椎横突综合征的远期疗效及镇痛机制，观察了第3腰椎横突综合征大鼠下丘脑、脊髓和外周血中一氧化氮合酶（NOS）和β-内啡肽（β-EP）的变化。最后三组进行模拟L-3横突综合征的手术。模拟手术后14天，分别进行电针和针刀治疗。结果：模拟手术组大鼠下丘脑、脊髓和外周血中NOS和PEP含量均明显高于正常组（P < 0. 05），而下丘脑、脊髓和外周血中NOS和PEP含量明显高于正常组（P < 0. 05）。电针组和针刀组NOS和β-EP含量明显低于模型组。电针组与针刀组比较差异无统计学意义（P > 0. 05）。结论：电针和针刀治疗可显著降低下丘脑、脊髓和外周血中NOS和β-EP的水平，减轻L-3横突综合征。
OBJECTIVE: To explore the long-term effects and pain relief mechanism of acupotomy by observing changes in nitric oxide synthase (NOS) and beta-endorphin (beta-EP) in the hypothalamus, spinal cord, and peripheral blood of rats with third lumbar vertebrae (L-3) transverse process syndrome.METHODS: Twenty-eight SD rats were randomly assigned to normal, model, electroacupuncture (EA), and acupotomy group. The last three groups were put through an operation to emulate L-3 transverse process syndrome. Fourteen days after the simulation operation, EA and acupotomy treatments were applied to the respective groups. Fifty-six days after the simulation operation, biochemistry tests and enzyme-linked immunosorbent assay were used to measure NOS and beta-EP in the hypothalamus, spinal cord, and peripheral blood.RESULTS: Rats with the simulation operation showed significantly higher levels of NOS and PEP in the hypothalamus, spinal cord, and peripheral blood than those in the normal group. The EA and acupotomy groups had significantly lower levels of NOS and beta-EP than those in the model group. There was no statistical difference between the EA and acupotomy groups.CONCLUSION: EA and acupotomy treatments significantly lowered NOS and (beta-EP) levels in the hypothalamus, spinal cord, and peripheral blood and alleviated L-3 transverse process syndrome.