Bio-ink for on-demand printing of living cells
Bio-ink for on-demand printing of living cells
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DOI：
10.1039/c2bm00114d
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发表时间：
2013-01-01
影响因子：
6.6
通讯作者：
Panhuis, Marc In Het
中科院分区：
文献类型：
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作者：
Ferris, Cameron J.;Gilmore, Kerry J.;Panhuis, Marc In Het
按需滴注生物打印允许活细胞的受控放置，并将有利于组织工程，药物筛选和毒理学领域的研究。我们表明，一种基于一种新型微凝胶悬浮液在含有表面活性剂的组织培养基中的生物墨水，可用于可重复地打印几种不同的细胞类型，从两种不同的市售按需滴印系统，在长时间的打印周期。生物墨水保持稳定的细胞悬浮液，防止通常阻碍细胞打印的细胞的沉降和聚集，同时满足甚至能够从多喷嘴商业喷墨打印头打印所需的严格的流体性质要求。打印技术的这一创新可能为多细胞结构和功能组织的生物制造铺平道路。
Drop-on-demand bioprinting allows the controlled placement of living cells, and will benefit research in the fields of tissue engineering, drug screening and toxicology. We show that a bio-ink based on a novel microgel suspension in a surfactant-containing tissue culture medium can be used to reproducibly print several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods. The bio-ink maintains a stable cell suspension, preventing the settling and aggregation of cells that usually impedes cell printing, whilst meeting the stringent fluid property requirements needed to enable printing even from many-nozzle commercial inkjet print heads. This innovation in printing technology may pave the way for the biofabrication of multi-cellular structures and functional tissue.