Catheterization of Pulmonary and Carotid Arteries for Concurrent Measurement of Mean Pulmonary and Systemic Arterial Pressure in Rat Models of Pulmonary Arterial Hypertension.
Catheterization of Pulmonary and Carotid Arteries for Concurrent Measurement of Mean Pulmonary and Systemic Arterial Pressure in Rat Models of Pulmonary Arterial Hypertension.
复制标题
DOI：
10.21769/bioprotoc.4737
复制
发表时间：
2023-08-20
期刊：
影响因子：
0.8
通讯作者：
中科院分区：
文献类型：
--
作者：
肺动脉高压（PH）是一组肺血管疾病，其中平均肺动脉压（mPAP）由于各种病理状况（包括肺动脉重塑、肺和心脏疾病或先天性疾病）而变得异常高。各种动物模型，包括小鼠和大鼠模型，已被用来重现在 PH 患者中观察到的 mPAP 升高。然而，测量和记录小动物的 mPAP 和平均全身动脉压 (mSAP) 需要显微外科手术和复杂的数据采集系统。在本文中，我们描述了用于测量大鼠 mPAP 的右心导管插入术 (RHC) 的手术程序。我们还解释了使用 PowerLab 数据采集系统同步测量 mPAP 和 mSAP 的颈动脉插管术。我们列举了暴露颈静脉和颈动脉以对这两条血管进行导管插入的手术步骤。我们列出了用于大鼠显微外科手术的工具，描述了制备导管的方法，并说明了将导管插入肺动脉和颈动脉的过程。最后，我们描述了用于记录 mPAP 和 mSAP 的 PowerLab 系统的校准和设置所涉及的步骤。这是第一个协议，其中我们详细解释了大鼠 RHC 的手术程序以及 mPAP 和 mSAP 的记录。我们相信该协议对于 PH 研究至关重要。缺乏动物处理训练的研究人员可以在大鼠中重现这种 RHC 显微外科手术，并在 PH 大鼠模型中测量 mPAP 和 mSAP。此外，该方案可能有助于掌握因其他疾病（例如心力衰竭、先天性心脏病、心脏瓣膜疾病和心脏移植）而进行的大鼠的 RHC。
Pulmonary hypertension (PH) is a group of pulmonary vascular disorders in which mean pulmonary arterial pressure (mPAP) becomes abnormally high because of various pathological conditions, including remodeling of the pulmonary arteries, lung and heart disorders, or congenital conditions. Various animal models, including mouse and rat models, have been used to recapitulate elevated mPAP observed in PH patients. However, the measurement and recording of mPAP and mean systemic arterial pressure (mSAP) in small animals require microsurgical procedures and a sophisticated data acquisition system. In this paper, we describe the surgical procedures for right heart catheterizations (RHC) to measure mPAP in rats. We also explain the catheterization of the carotid artery for simultaneous measurement of mPAP and mSAP using the PowerLab Data Acquisition system. We enumerate the surgical steps involved in exposing the jugular vein and the carotid artery for catheterizing these two blood vessels. We list the tools used for microsurgery in rats, describe the methods for preparing catheters, and illustrate the process for inserting the catheters in the pulmonary and carotid arteries. Finally, we delineate the steps involved in the calibration and setup of the PowerLab system for recording both mPAP and mSAP. This is the first protocol wherein we meticulously explain the surgical procedures for RHC in rats and the recording of mPAP and mSAP. We believe this protocol will be essential for PH research. Investigators with little training in animal handling can reproduce this microsurgical procedure for RHC in rats and measure mPAP and mSAP in rat models of PH. Further, this protocol is likely to help master RHC in rats that are performed for other conditions, such as heart failure, congenital heart disease, heart valve disorders, and heart transplantation.
选择性PGI2受体激动剂selexipag可以改善SUGEN 5416/缺氧诱导的大鼠肺动脉高压。
影响因子：
3.7
作者：
Honda Y;Kosugi K;Fuchikami C;Kuramoto K;Numakura Y;Kuwano K
通讯作者：
Kuwano K
影响因子：
1.7
作者：
Lachant DJ;Meoli DF;Haight D;Lyons JA;Swarthout RF;White RJ
通讯作者：
White RJ
DOI：
10.3390/ph15101242
发表时间：
2022-10-10
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--