Analysis of the activity and biological control efficacy of the Bacillus subtilis strain Bs-1 against Meloidogyne incognita
Analysis of the activity and biological control efficacy of the Bacillus subtilis strain Bs-1 against Meloidogyne incognita
复制标题
枯草芽孢杆菌Bs-1对南方根结线虫活性及生防效果分析
DOI：
10.1016/j.cropro.2019.04.021
复制
发表时间：
2019-08-01
期刊：
影响因子：
2.8
通讯作者：
Xie, Bingyan
中科院分区：
文献类型：
--
作者：
Cao, Hongyi;Jiao, Yang;Xie, Bingyan
根结线虫是一种严重的土传病原菌，每年在世界范围内给蔬菜作物造成巨大的危害和经济损失。因此，迫切需要寻找新的杀线虫剂或生物防治剂。本研究从南方根结线虫根际土壤中筛选到一株枯草芽孢杆菌Bs-1菌株，该菌株对南方根结线虫具有较强的杀线虫作用，并对南方根结线虫卵的孵化有抑制和驱避作用。根据发酵测定评价杀线虫活性，并且第二阶段幼虫（J2 s）对Bs-1发酵处理的死亡率为100%。杀卵试验结果表明，菌株Bs-1发酵液对卵的孵化率也有明显的降低作用。在盆栽和田间试验中，Bs-1发酵液均能显著减少黄瓜根癌，促进黄瓜生长。Bs-1发酵液对J2的驱避试验表明，大部分J2对Bs-1发酵液和发酵液上清有明显的驱避作用，而对黄瓜根浸提液有明显的引诱作用。气相色谱-质谱联用（GC-MS）分析结果表明，菌株Bs-1产生的挥发性有机物（VOCs）成分复杂多样，有酮类、酸类、烷基类、硫化物和杂环类等，说明菌株B.枯草芽孢杆菌Bs-1菌株对M.可作为一种潜在的生物防治剂来抑制M.无名氏
Root-knot nematodes are severe soil-borne pathogens that cause great damage to vegetable crops and economic losses all over the world annually. Therefore, there is an urgent need to seek novel nematicides or biological control agents. In this study, a bacterium strain, the Bacillus subtilis strain Bs-1, was obtained from rhizospheric soil, and had strong nematicidal effects and caused egg hatching inhibition and repellence of Meloidogyne incognita. Nematicidal activity was evaluated according to fermentation assays, and the mortality rate of second-stage juveniles (J2s) to the Bs-1 fermentation treatment was 100%. The results of ovicidal experiments indicated that fermentation of the strain Bs-1 also had a strong effect on decreasing the egg hatching rate. In both pot and field experiments, Bs-1 fermentation was able to significantly reduce root galls and promote the growth of cucumber. A repellence test of Bs-1 fermentation showed that a majority of J2s were obviously repelled by the Bs-1 fermentation and supernatant, but were attracted by cucumber root extracts. The gas chromatography-mass spectrometry (GC-MS) results showed that the volatile organic components (VOCs) produced by the strain Bs-1 were complex and diverse, such as ketones, acids, alkyls, sulfide and heterocyclic compounds and so on. These results revealed that the B. subtilis strain Bs-1 provides strong suppression against M. incognita and could be used as a potential biological control agent to suppress M. incognita.