EFFECT OF PROTEIN-SYNTHESIS INHIBITORS AND METABOLIC BLOCKERS ON THE PRODUCTION OF PLACENTAL PROTEINS BY THE INVITRO PERFUSED HUMAN-PLACENTA
EFFECT OF PROTEIN-SYNTHESIS INHIBITORS AND METABOLIC BLOCKERS ON THE PRODUCTION OF PLACENTAL PROTEINS BY THE INVITRO PERFUSED HUMAN-PLACENTA
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DOI：
10.1159/000293763
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发表时间：
1988-01-01
影响因子：
2.1
通讯作者：
SCHNEIDER, H
中科院分区：
文献类型：
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作者：
BERSINGER, NA;MALEK, A;SCHNEIDER, H
研究了新鲜递送的人足月胎盘在体外双重灌注期间产生和释放胎盘蛋白的能力。在单独的封闭循环中灌注器官，并定期从母体和胎儿循环中取出等份培养基。在这些培养基中以及在灌注之前和之后的胎盘组织中定量胎盘蛋白人绒毛膜促性腺激素（HCG）、人胎盘催乳素（HPL）、妊娠特异性β 1-糖蛋白（SP1）和妊娠相关血浆蛋白A（PAPP-A）。发现上述四种蛋白质在灌注间隔（90分钟至3小时）期间合成，而妊娠相关α 2-糖蛋白和催乳素仅被洗出。当放线菌酮、嘌呤霉素、碘乙酸或2，4-二硝基苯酚加入到灌流培养基中时，HCG、HPL、SP1和PAPP-A的平均产量降低。在这四种抗代谢物中，碘乙酸对总释放和净合成的影响最严重。它的结论是，上述四种蛋白质合成从头灌注胎盘在没有母体组织，这种合成是能量依赖性的。
The capacity of the freshly delivered human term placenta to produce and release placental proteins during in vitro dual perfusion was investigated. The organ was perfused in separate closed circulations and aliquots of medium were taken at regular intervals from both maternal and fetal circuits. The placental proteins human chorionic gonadotrophin (HCG), human placental lactogen (HPL), pregnancy-specific .beta.1-glycoprotein (SP1), and pregnancy-associated plasma protein A (PAPP-A) were quantified in these media as well as in the placental tissue before and after the perfusion. It was found that the four above-mentioned proteins were synthesized during the perfusion interval (90 min to 3 h) while pregnancy-associated .alpha.2-glycoprotein and prolactin were only washed out. The mean production of HCG, HPL, SP1, and PAPP-A was decreased when either cycloheximide, puromycin, iodoacetic acid, or 2,4-dinitrophenol had been added to the perfusing medium. Amongst these four antimetabolites iodoacetic acid most severely affected both the total release and net synthesis. It is concluded that the above four proteins are synthesized de novo by the perfused placenta in the absence of maternal tissue and that this synthesis is energy-dependent.