Baculoviruses: high-level expression in insect cells.
Baculoviruses: high-level expression in insect cells.
复制标题
杆状病毒：在昆虫细胞中高水平表达。
DOI：
10.1016/s0959-437x(05)80348-x
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发表时间：
1993
影响因子：
4
通讯作者：
Miller,LK
中科院分区：
文献类型：
--
作者：
Miller,LK
杆状病毒继续作为昆虫细胞中高水平表达真核基因的主力载体；然而，许多研究人员还通过利用病毒及其宿主细胞的独特性质，发现了这些载体的新用途。同时，构建和利用这些载体所涉及的技术正在不断改进，从而减少了构建表达载体所需的时间和精力。
Baculoviruses continue to serve as workhorse vectors for the high-level expression of eukaryotic genes in insect cells; however, numerous researchers are also finding novel uses for these vectors by taking advantage of the unique nature of the viruses and their host cells. Concurrently, the technology involved in constructing and utilizing these vectors is being improved so that the time and effort required to construct expression vectors are reduced.