Expression of integrin subunits α2, α3, α5, αv, β1, β3 and β4 in different histological types of ameloblastoma compared with dental germ, dental lamina and adult lining epithelium
Expression of integrin subunits α2, α3, α5, αv, β1, β3 and β4 in different histological types of ameloblastoma compared with dental germ, dental lamina and adult lining epithelium
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DOI：
10.1111/j.1601-0825.2004.01028.x
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发表时间：
2004-09-01
期刊：
影响因子：
3.8
通讯作者：
de Araújo, VC
中科院分区：
文献类型：
--
作者：
Modolo, F;Martins, MT;de Araújo, VC
目的：分析整合素在不同组织学类型成釉细胞瘤中的表达和分布，并与牙胚、牙板和成人衬里上皮进行比较。材料和方法：采用链霉亲和素-生物素免疫组织化学方法和抗整合素α 2、α 3、α 5、α v、β 1、β 3和β 4抗体对石蜡包埋标本的3微米切片进行评价。所有整合素存在于所有标本中，表现出不同的模式。在滤泡性成釉细胞瘤中，整联蛋白染色在外周较强，而整联蛋白α 2在中央细胞中不存在。胶质瘤性成釉细胞瘤表现出类似的模式，在化生细胞中整合素α 3、α 5、α v、β 1和β 4阳性染色。在单囊中，整联蛋白染色是均匀的，除了整联蛋白α 5和β 3在上层显示较弱的染色。在丛状成釉细胞瘤中，牙胚和牙板整合素染色均匀。在成人衬里上皮中，整合素α 2、α 5和β 4的染色仅限于基底层，而整合素α v和β 3存在于基底层和副基底层，整合素α 3和β 1存在于上层。恶性肿瘤，滤泡型和单囊型成釉细胞瘤显示与成人衬里上皮相似的整合素染色模式，而丛状成釉细胞瘤显示与牙胚相似的整合素染色模式和叶片。
OBJECTIVE: To analyze integrin expression and distribution in different histological types of ameloblastoma, compared with dental germ, dental lamina and adult lining epithelium.MATERIALS AND METHODS: Three-micrometer sections from paraffin-embedded specimens were evaluated employing a streptavidin-biotin immunohistochemical method and anti-integrin alpha2, alpha3, alpha5, alphav, beta1, beta3 and beta4 antibodies.RESULTS: All integrins were present in all specimens, exhibiting different patterns. In follicular ameloblastoma, the integrin staining was stronger in the periphery while integrin alpha2 was not present in the central cells. Acanthomatous ameloblastoma showed a similar pattern, with positive staining for integrins alpha3, alpha5, alphav, beta1 and beta4 in the metaplastic cells. In the unicystic, integrin staining was uniform except for integrins alpha5 and beta3 which showed weaker staining in the upper layers. In the plexiform ameloblastoma, dental germ and lamina integrin staining was uniform. In the adult lining epithelium, staining for integrins alpha2, alpha5 and beta4 was confined to the basal layer, while integrins alphav and beta3 were present in the basal and parabasal, with integrins alpha3 and beta1 in the upper layers.CONCLUSION: Acanthomatous, follicular and unicystic ameloblastomas showed integrin staining patterns similar to the adult lining epithelium while the plexiform ameloblastoma was similar to the dental germ and lamina.