Cloning and characterization of two genes encoding Schistosoma mansoni elastase.
Cloning and characterization of two genes encoding Schistosoma mansoni elastase.
复制标题
DOI：
10.1016/0166-6851(95)02502-2
复制
发表时间：
1995-12
影响因子：
1.5
通讯作者：
C. Pierrot;A. Capron;J. Khalife
中科院分区：
文献类型：
--
作者：
C. Pierrot;A. Capron;J. Khalife
分离并测序了一个编码先前鉴定的曼氏血吸虫酶的基因组DNA克隆（EL1）。该基因含有约140和604个核苷酸的3个外显子和2个内含子。它在近端5′区含有潜在的调控序列。同时利用s。在曼索基因组DNA中，鉴定出第二弹性酶基因（EL），其内含子长度分别为131和254个核苷酸，位于与EL1相同的位置。所得氨基酸序列与EL1的同源性为79%。值得注意的是，逆转录pcr扩增显示只有EL1的存在。这可能是由于第二个基因的mRNA水平较低和/或引物与两个转录本的竞争所致。[核苷酸序列数据提交至EMBL基因库，登录号为U31768和U31769]。
A genomic DNA clone (EL1) coding a previously identifiedSchistosoma mansonielastase was isolated and sequenced. The gene contained 3 exons and 2 introns of about 140 and 604 nucleotides. It contained potential regulatory sequences in the proximal 5′-region. While confirming the sequence of this elastase using PCR amplification ofS. mansonigenomic DNA, a 2nd elastase gene (EL) was identified which had 2 introns, 131 and 254 nucleotides in length, located at the same positions when compared to EL1. The deduced amino acid sequence showed 79% identity with EL1. It was noted that reverse transcription-PCR amplification revealed the presence of only EL1. It is suggested that this may be due to the low level of mRNA for the 2nd gene and/or to the competition of primers with the 2 transcripts. [Nucleotide sequence data submitted to the EMBL GeneBank, accession numbers U31768 and U31769].