Hydrophilic interaction ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides, nucleosides and nucleobases in Ziziphus plants
Hydrophilic interaction ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides, nucleosides and nucleobases in Ziziphus plants
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DOI：
10.1016/j.chroma.2013.05.074
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发表时间：
2013-08-02
影响因子：
4.1
通讯作者：
Shang, Erxin
中科院分区：
文献类型：
--
作者：
Guo, Sheng;Duan, Jin-ao;Shang, Erxin
采用亲水作用超高效液相色谱-三重四极杆串联质谱（HILIC-UHPLC-TQ-MS/MS）技术，在多反应监测（MRM）模式下，建立了一种快速、灵敏测定枣属植物中20种核苷、核苷和核苷酸的分析方法。在优化的色谱条件下，20种目标化合物在粒径小于2 μ m的Amide柱上10 min内得到了良好的分离，20种目标化合物的检出限和定量限分别为0.11-3.12 ng mL（-1）和0.29-12.48 ng mL（-1）。首次报道了HILIC-UHPLC-TQ-MS/MS法同时分析药用植物中的核苷、核苷和核苷酸，该方法具有良好的线性、精密度、重复性和准确度。该方法已成功应用于枣属植物（Ziziphus plants，Z.巴湾spinosa和Z mauritiana）样品。结果表明，枣属植物果实和叶片中含有丰富的核苷、碱基和核苷酸，可作为保健食品资源。本研究结果表明，HILIC-UHPLC-TQ-MS/MS方法可作为一种有用的工具，用于以核苷酸、核苷和核碱基为标记物的枣属植物样品以及其他药用植物或食品样品的质量评价。(C)2013爱思唯尔有限公司版权所有。
In this study, a rapid and sensitive analytical method was developed for the determination of 20 nucleobases, nucleosides and nucleotides in Ziziphus plants at trace levels by using hydrophilic interaction ultra-high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UHPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under the optimized chromatographic conditions, good separation for 20 target compounds were obtained on a UHPLC Amide column with sub-2 mu m particles within 10 min. The overall LODs and LOQs were between 0.11-3.12 ng mL(-1) and 0.29-12.48 ng mL(-1) for the 20 analytes, respectively. It is the first report about simultaneous analysis of nucleobases, nucleosides and nucleotides in medicinal plants using HILIC-UHPLC-TQ-MS/MS method, which affords good linearity, precision, repeatability and accuracy. The developed method was successfully applied to Ziziphus plant (Z jujuba, Z. jujuba var. spinosa and Z mauritiana) samples. The analysis showed that the fruits and leaves of Ziziphus plants are rich in nucleosides and nucleobases as well as nucleotides, and could be selected as the healthy food resources. Our results in present study suggest that HILIC-UHPLC-TQ-MS/MS method could be employed as a useful tool for quality assessment of the samples from the Ziziphus plants as well as other medicinal plants or food samples using nucleotides, nucleosides and nucleobases as markers. (C) 2013 Elsevier B.V. All rights reserved.