BOVINE 1-2-CELL EMBRYO DEVELOPMENT USING A SIMPLE MEDIUM IN 3 OVIDUCT EPITHELIAL-CELL COCULTURE SYSTEMS
BOVINE 1-2-CELL EMBRYO DEVELOPMENT USING A SIMPLE MEDIUM IN 3 OVIDUCT EPITHELIAL-CELL COCULTURE SYSTEMS
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DOI：
10.1095/biolreprod43.1.97
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发表时间：
1990-07-01
影响因子：
3.6
通讯作者：
FOOTE, RH
中科院分区：
文献类型：
--
作者：
ELLINGTON, JE;CARNEY, EW;FOOTE, RH
这些研究旨在开发一种使用简单培养基的共培养系统，以促进1细胞牛胚胎从8-16细胞期发育到桑椹胚和囊胚期。从牛输卵管上皮细胞（BOEC）制备用于共培养的单层。从超数排卵的奶牛中手术收集体内受精的1-2细胞胚胎和卵子（384个）。在实验1中，在简单的无葡萄糖和无血清培养基（CZB）中共培养的胚胎发育的胚胎质量评分上级在Ham's F-10与血清中共培养的胚胎，并且比在CMRL-1066与血清中共培养的胚胎发育超过8-16个细胞的百分比更大（p < 0.05）。在实验2中，与新鲜BOEC单层共培养的胚胎平均比与冻融BOEC单层或BOEC条件培养基共培养的胚胎多（p < 0.05）个细胞。在培养的前48小时内，简单培养基中不含葡萄糖，到培养第5.5天（供体动情周期的第6.5天）时，比在始终存在葡萄糖的相同培养基中的胚胎更多（p < 0.01）。在实验3中，在BOEC共培养物中比在条件培养基中倾向于孵化更多的胚胎（p < 0.01）。这些结果表明，化学简单的培养基与新鲜的BOEC单层可以提供一个显着的好处，早期牛胚胎的共培养。
These studies were designed to develop a coculture system using a simple medium to promote development of 1-cell bovine embryos through the 8-16-cell stage to morula and blastocyst stages. Monolayers for coculture were prepared from bovine oviduct epithelial cells (BOEC). In vivo-fertilized 1-2 cell embryos and ova (384) were surgically collected from superovulated cows. In Experiment 1, embryos cocultured in a simple glucose-free and serum-free medium (CZB) developed with superior scores of embryo quality than embryos cocultured in Ham''s F-10 with serum, and a greater percentage developed past 8-16 cells than embryos cocultured in CMRL-1066 with serum (p < 0.05). In Experiment 2, embryos cocultured with fresh BOEC monolayers averaged more (p < 0.05) cells than did embryos in coculture with frozen-thawed BOEC monolayers or in BOEC-conditioned medium. Without glucose in the simple medium for the first 48 h of culture, more embryos blastulated (p < 0.01) by Day 5.5 of culture (Day 6.5 of donor''s estrous cycle) than embryos in the same medium with glucose present throughout. In Experiment 3, more embryos tended to hatch in BOEC coculture (p < 0.01) than in conditioned medium. These results show that a chemically simple medium with fresh BOEC monolayers can provide a significant benefit for coculture of early bovine embryos.