Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation.
Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation.
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DOI：
10.1016/j.devcel.2018.11.031
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发表时间：
2019-01
影响因子：
11.8
通讯作者：
Koichi Toyokura;T. Goh;Hidefumi Shinohara;Akinori Shinoda;Y. Kondo;Yoshie Okamoto;Takeo Uehara;K. Fujimoto;Y. Okushima;Y. Ikeyama;K. Nakajima;T. Mimura;M. Tasaka;Y. Matsubayashi;H. Fukaki
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作者：
Koichi Toyokura;T. Goh;Hidefumi Shinohara;Akinori Shinoda;Y. Kondo;Yoshie Okamoto;Takeo Uehara;K. Fujimoto;Y. Okushima;Y. Ikeyama;K. Nakajima;T. Mimura;M. Tasaka;Y. Matsubayashi;H. Fukaki
在植物中，侧根（LR）的位置取决于生长素诱导的起始位点。负责 LR 启动的高生长素反应域延伸到多个细胞，但只有一对中柱鞘细胞（LR 创始人细胞）会发育成 LR。在这项工作中，我们确定了通过侧抑制控制 LR 形成的信号级联。它包含肽激素 TARGET OF LBD SIXTEEN 2 (TOLS2)、其受体 RLK7 和下游转录因子 PUCHI。TOLS2 在 LR 创始人细胞中表达并抑制 LR 启动。生长素响应性 DR5:LUCIFERASE 报告基因表达的延时成像显示，即使在野生型中，偶尔也有两对 LR 创始细胞非常接近，其中之一仅短暂存在，并以 RLK7 依赖性方式消失。我们建议通过肽激素受体级联选择 LR 创始细胞，以确保适当的 LR 间距。
In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI.TOLS2is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsiveDR5:LUCIFERASEreporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.