Inhibition of 2,4-dichlorophenoxyacetic Acid-stimulated elongation of pea stem segments by a xyloglucan oligosaccharide.
Inhibition of 2,4-dichlorophenoxyacetic Acid-stimulated elongation of pea stem segments by a xyloglucan oligosaccharide.
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木葡聚糖寡糖抑制 2,4-二氯苯氧基乙酸刺激的豌豆茎段伸长。
作者：
W. York;A. Darvill;P. Albersheim
从悬浮培养的西卡莫尔（Acer pseudoplatanus）细胞的可溶性胞外多糖中分离木葡聚糖，用从绿色木霉培养液中纯化的内切-β-1，4-葡聚糖酶消化。一个nonascuridide丰富的生物凝胶P-2级分的消化抑制2，4-二氯苯氧乙酸刺激的黄化豌豆茎段的伸长。该寡糖级分的抑制活性表现出明确的最佳浓度，在10（-2）和10（-1）微克/毫升之间。同样的木葡聚糖消化物的另一部分，富含结构相关的七糖，在相似的浓度下，没有显著抑制伸长。
Xyloglucan, isolated from the soluble extracellular polysaccharides of suspension-cultured sycamore (Acer pseudoplatanus) cells, was digested with an endo-beta-1,4-glucanase purified from the culture fluid of Trichoderma viride. A nonasaccharide-rich Bio-Gel P-2 fraction of this digest inhibited 2,4-dichlorophenoxyacetic-acid-stimulated elongation of etiolated pea stem segments. The inhibitory activity of this oligosaccharide fraction exhibited a well-defined concentration optimum between 10(-2) and 10(-1) micrograms per milliliter. Another fraction of the same xyloglucan digest, rich in a structurally related heptasaccharide, did not, at similar concentrations, significantly inhibit the elongation.