Infrared laser-mediated gene induction in targeted single cells in vivo
Infrared laser-mediated gene induction in targeted single cells in vivo
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DOI：
10.1038/nmeth.1278
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发表时间：
2009-01-01
期刊：
影响因子：
48
通讯作者：
Yuba, Shunsuke
中科院分区：
文献类型：
--
作者：
Kamei, Yasuhiro;Suzuki, Motoshi;Yuba, Shunsuke
我们开发了红外激光诱发基因操作器(IR-LEGO)，这是一种优化的显微镜系统，可以在不造成光化学损伤的情况下加热细胞。红外辐射以一种功率依赖的方式引起体外微环境的可重复的温度漂移。当应用于活的秀丽隐杆线虫时，IR-LEGO以比440 nm染料激光更有效且危害更小的方式在目标单细胞中诱导热休克介导的转基因表达，并引发生理相关的表型反应。
We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.