Using wettability and interfacial tension to handle droplets of magnetic beads in a micro-chemical-analysis system
Using wettability and interfacial tension to handle droplets of magnetic beads in a micro-chemical-analysis system
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DOI：
10.1016/j.snb.2005.01.029
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发表时间：
2006-01-17
影响因子：
8.4
通讯作者：
Sato, K
中科院分区：
文献类型：
--
作者：
Shikida, M;Takayanagi, K;Sato, K
我们开发了一种微化学分析系统的磁珠-液滴处理机制，使我们能够可靠地操纵磁珠液滴。不同液体之间的润湿性和界面张力的差异可以防止珠子粘在玻璃表面。我们还开发了提取和熔炼珠滴的机制，并证实了将提取和熔炼装置结合起来可以有效地凝聚和冲洗珠。含有珠团的萃取液滴的平均液体体积为30 nl，与紧密排列的珠团结构中空隙的液体体积基本相同。通过我们的液滴提取和融合工艺，提取的液滴可以用新鲜的液滴稀释1600倍。该系统的主要优点是它不需要机械装置，如泵，阀门和过滤器来处理溶液。(c) 2005 Elsevier B.V.版权所有
We developed a beads-droplet-handling mechanism for micro-chemical-analysis systems that enables us to manipulate magnetic-bead droplets reliably. Differences in wettability and the interfacial tension between different liquids prevent the beads from sticking to the glass surfaces. We also developed mechanisms for extracting and fusing bead droplets and confirmed that beads can be effectively condensed and rinsed by combining extraction and fusion units. The average liquid volume of an extracted droplet containing a bead cluster was 30 nl, which is almost the same of that of the void space in a closely packed bead-cluster structure. An extracted droplet can be diluted 1600-fold with our droplet extraction and fusion process by using a fresh droplet. The primary advantage of the system is that it does not need mechanical devices such as pumps, valves, and filters for handling a solution. (c) 2005 Elsevier B.V. All rights reserved.