LISTERIOLYSIN-O IS A TARGET OF THE IMMUNE-RESPONSE TO LISTERIA-MONOCYTOGENES
LISTERIOLYSIN-O IS A TARGET OF THE IMMUNE-RESPONSE TO LISTERIA-MONOCYTOGENES
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DOI：
10.1084/jem.175.6.1467
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发表时间：
1992-06-01
影响因子：
15.3
通讯作者：
HINRICHS, DJ
中科院分区：
文献类型：
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作者：
BOUWER, HGA;NELSON, CS;HINRICHS, DJ
抗单核细胞增生李斯特菌（Listeria monocytogenes，Lm）保护性免疫的免疫学机制尚不清楚，但Lm免疫供体的T淋巴细胞可过继性转移抗Lm免疫。Lm免疫细胞被认为产生巨噬细胞激活淋巴因子，这导致最终的巨噬细胞依赖性根除细菌。越来越多的证据表明，对Lm的免疫力仅存在于CD 8 + T细胞亚群中。Lm免疫CD 8 + T细胞的功能可能是将非专职吞噬细胞中的Lm释放到等待活化的巨噬细胞群中。进行本研究以确定作为Lm致病性的基本决定因素的嗜酸乳杆菌溶血素O（LLO）是否也是抗嗜酸乳杆菌免疫应答的靶标。我们已经发现，用LLO+ Lm菌株感染的靶细胞被Lm-免疫细胞毒性细胞裂解，而用LLO-Lm突变体感染或用热灭活的Lm制剂脉冲的靶细胞不被Lm-免疫效应细胞裂解。我们已经使用了表达LLO基因产物的枯草芽孢杆菌（Bs）构建体，并且发现用LLO+ Bs构建体感染的靶细胞被抗真菌细胞毒性细胞裂解。抗真菌细胞毒性应答靶向LLO，因为我们还使用了表达产气荚膜梭菌溶素（PLO）基因产物的Bs构建体，并发现用PLO+ Bs感染的靶细胞不被抗真菌细胞毒性效应细胞裂解。这些数据有力地表明，LLO是抗结核免疫的靶抗原，并且可能代表在对Lm的免疫应答的表达期间的主导靶标。
The immunologic mechanism of protective immunity to the intracellular parasite Listeria monocytogenes (Lm) is not well understood, however, antilisterial immunity can be adoptively transferred with T lymphocytes from Lm-immune donors. The Lm-immune cells are believed to produce macrophage-activating lymphokines, which leads to the eventual macrophage-dependent eradication of the bacterium. Increasing evidence suggests that immunity to Lm resides exclusively within the CD8+ T cell subset. It is possible that the Lm-immune CD8+ T cells function to release sequestered Lm from nonprofessional phagocytes to awaiting activated macrophage populations. This study was conducted to determine if listeriolysin O (LLO), which is an essential determinant of Lm pathogenicity, is also a target of the antilisterial immune response. We have found that target cells infected with a LLO+ Lm strain are lysed by Lm-immune cytotoxic cells, whereas target cells infected with a LLO- Lm mutant, or pulsed with a heat-killed Lm preparation, are not lysed by the Lm-immune effector cells. We have used a Bacillus subtilis (Bs) construct that expresses the LLO gene product and found that target cells infected with the LLO+ Bs construct are lysed by antilisterial cytotoxic cells. The antilisterial cytotoxic response is targeted against LLO, in that we have also used a Bs construct that expresses the perfringolysin (PLO) gene product and found that target cells infected with the PLO+ Bs are not lysed by antilisterial cytotoxic effector cells. These data strongly suggest that LLO is a target antigen of antilisterial immunity and may represent the dominant target during the expression of the immune response to Lm.