Downregulation of SKA1 Gene Expression Inhibits Cell Growth in Human Bladder Cancer
Downregulation of SKA1 Gene Expression Inhibits Cell Growth in Human Bladder Cancer
复制标题
SKA1基因表达下调抑制人膀胱癌细胞生长
DOI：
10.1089/cbr.2014.1715
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发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Xie, Hailong
中科院分区：
文献类型：
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作者：
Tian, Feng;Xing, Xiaoxiao;Xie, Hailong
纺锤体和着丝粒相关蛋白1（SKA 1）是着丝粒微管结合复合物的一个组成部分，对染色体的正常分离至关重要。最近，SKA 1已被证明参与几种人类癌症的恶性进展。然而，它在膀胱癌中的作用仍然未知。为了评估SKA 1在膀胱癌细胞中的功能，作者采用RNA干扰慢病毒系统来消除其在BT5637和T-24膀胱癌细胞中的表达。SKA 1敲低后，两种细胞系的细胞增殖均显著降低。此外，殖民地形成能力受到SKA 1沉默的损害。流式细胞仪分析显示SKA 1的缺失导致细胞周期停滞在S期。此外，T-24细胞中SKA 1的敲除明显下调CDK 4和Cyclin D1的表达，减轻ERK 2和AKT的激活，有利于细胞生长抑制。这些结果表明，SKA 1基因的敲减可以有效抑制膀胱癌细胞的体外增殖，慢病毒介导的SKA 1基因沉默可能成为膀胱癌基因治疗的新策略。
Spindle and kinetochore-associated protein 1 (SKA1), a component of microtubule-binding complex of kinetochore, is essential for proper chromosome segregation. Recently, SKA1 has been shown to be involved in malignant progression of several human cancers. However, its role in bladder cancer is still unknown. To evaluate the function of SKA1 in bladder cancer cells, the authors employed an RNA interference lentivirus system to deplete its expression in both BT5637 and T-24 bladder cancer cells. The cell proliferation was significantly decreased in both cell lines after SKA1 knockdown. Moreover, the colony formation capacity was impaired by SKA1 silencing. Flow cytometry analysis showed that depletion of SKA1 led to cell cycle arrest at S phase. Furthermore, knockdown of SKA1 in T-24 cells obviously downregulated the expressions of CDK4 and Cyclin D1, and alleviated the activations of ERK2 and AKT, conducive to cell growth inhibition. These findings suggested that knockdown of SKA1 could potently suppress bladder cancer cell proliferation in vitro and lentivirus-mediated silencing of SKA1 might serve as a novel strategy for gene therapy of bladder cancer.