Pro-inflammatory and T cell inhibitory cytokines are secreted at high levels in tumor cell cultures of human renal cell carcinoma
Pro-inflammatory and T cell inhibitory cytokines are secreted at high levels in tumor cell cultures of human renal cell carcinoma
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DOI：
10.1159/000019821
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发表时间：
1999-01-01
期刊：
影响因子：
23.4
通讯作者：
Lindemann, A
中科院分区：
文献类型：
--
作者：
Lahn, M;Fisch, P;Lindemann, A
目的：本研究的目的是评估人肾细胞癌（RCC）细胞因子的分泌，并确定有助于肿瘤细胞的免疫调节作用的细胞因子。方法：采用ELISA法检测原代肿瘤细胞（PTCC）和相应细胞系（CL）培养上清中细胞因子的分泌。通过形态学、免疫细胞化学和流式细胞术分析肿瘤细胞的特征。结果：检测了27例PTCC及其相应的RCC（3/27）的细胞因子分泌。我们发现RCC主要产生促炎性和T细胞抑制性细胞因子，如IL-8、IL-6、GM-CSF、INF-α、IL-10和TGF-β（1）。CL适应无血清培养基，这可能被证明是一个有用的工具，在未来的研究细胞因子分泌在RCC。此外，我们使用γ δ和α β T细胞克隆来评估RCC肿瘤细胞的免疫调节作用，发现RCC主要激活γ δ T细胞。结论：我们的数据表明，RCC产生大量促炎细胞因子和T细胞抑制细胞因子，这些细胞因子可能会影响宿主的免疫反应，尤其是肿瘤特异性细胞毒性T细胞。
Objectives: The objectives of this study were to assess cytokine secretion in human renal cell carcinoma (RCC) and to identify cytokines contributing to the immunomodulatory effect of tumor cells. Methods: Cytokine secretion in the supernatant of primary tumor cell cultures (PTCC) and corresponding cell lines (CL) was assayed using ELISA. Tumor cells were characterized by morphology, immunocytochemistry, and flow-cytometric analysis. Tumor-cell-induced T cell activation was determined by coculture of gamma delta and alpha beta T cell clones with tumor CL, Results: We assessed the cytokine secretion of tumor cells from 27 PTCC and their corresponding CL (3/27) of RCC. We found that RCC predominantly produced both pro-inflammatory and T-cell-inhibitory cytokines, such as IL-8, IL-6, GM-CSF, INF-alpha, IL-10 and TGF-beta(1). CL were adapted to serum-free medium which may prove as a useful tool in future studies of cytokine secretion in RCC. In addition, we used gamma delta and alpha beta T cell clones to assess the immunomodulatory effect of tumor cells from RCC and found that predominantly gamma delta T cells were activated by RCC. Conclusions: Our data suggest that RCC produce large amounts of both pro-inflammatory and T-cell-inhibitory cytokines that potentially could influence the immune response of the host, especially tumor-specific cytotoxic T cells.