BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype
BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype
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DOI：
10.1080/14653240701358445
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发表时间：
2007-01-01
期刊：
影响因子：
4.5
通讯作者：
Kapp, U.
中科院分区：
文献类型：
--
作者：
Kaiser, S.;Hackanson, B.;Kapp, U.
背景成人骨髓间充质基质细胞(MSC)具有成纤维细胞样形态、贴壁生长和向脂肪细胞、骨细胞、软骨细胞、心肌细胞和神经前体细胞分化的能力。这些细胞在体外培养后，表达细胞表面分子CD44、CD90、SH2和SH3，CD34和造血标记物CD45阴性。方法对骨髓单个核细胞进行CD34(+)、CD34(-)、CD45(+)、CD34(+)、CD45(高+)CD34(-)、CD45(中、低+)CD34(-)、CD45(-)CD34(-)亚群培养，并对其进行生长分析。虽然大多数来源于CD45(+)CD34(+)亚群。各组分的MSC均可分化为脂肪细胞和骨细胞。此外，CD34(+)和CD34(-)组分的MSC可分化为软骨细胞。经体外培养后，两组分的MSC具有相同的表型，CD34和CD45均为阴性。
Background Mesenchymal stromal cells (MSC) isolated from adult human BM are characterized by their fibroblast-like morphology, adherent growth and capacity to differentiate into adipocytes, osteocytes, chondrocytes, cardiomyocytes and neuroprogenitors. After culturing these cells in vitro, they express the cell-surface molecules CD44, CD90, SH2 and SH3, and are negative for CD34 and the hematopoietic marker CD45. The aim of this study was to characterize the in vivo phenotype of MSC relative to the expression of CD34 and CD45.Methods BM mononuclear cells were stained with Ab against both molecules and separated into the CD34(+), CD34(-), CD45(+) CD34(+), CD45(high+) CD34(-), CD45(med,low+) CD34(-) and CD45(-) CD34(-) subpopulations, which were then cultured under the same conditions and analyzed for growth of MSC.Results A small population of MSC arose from the CD45(+) CD34(-) fraction, although the majority was obtained from the CD45(+) CD34(+) subpopulation. MSC from all fractions could be differentiated into adipocytes and osteocytes. In addition, MSC from the CD34(+) and CD34(-) fractions were shown to differentiate into chondrocytes. After in vitro culture, MSC from both fractions possessed the same phenotype, which was negative for CD34 and CD45.Discussion MSC from the CD45(+) CD34(+) fraction change their phenotype under in vitro conditions.