Electrophoretic analysis of human herpesvirus 6 polypeptides immunoprecipitated from infected cells with human sera.
Electrophoretic analysis of human herpesvirus 6 polypeptides immunoprecipitated from infected cells with human sera.
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对用人血清从感染细胞中免疫沉淀的人疱疹病毒 6 多肽进行电泳分析。
DOI：
10.1093/infdis/163.1.29
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Salahuddin,SZ
中科院分区：
文献类型：
--
作者：
Balachandran,N;Tirawatnapong,S;Pfeiffer,B;Ablashi,DV;Salahuddin,SZ
检测了健康成人和AIDS、慢性疲劳综合征、霍奇金病和干燥综合征患者血清中引起人抗体应答的人疱疹病毒6型（HHV-6）蛋白。免疫荧光法检测HHV-6 IgG抗体滴度为1：10 ~ 1：1280。用pSS]蛋氨酸、[~ 3 H]葡糖胺和uSI标记HHV-6感染和未感染细胞的裂解物，用血清免疫沉淀并电泳分析。IF滴度≥ 1：20的血清免疫沉淀>20个[35 S]蛋氨酸标记的HHV-6多肽，分子量为26-180 kDa。至少有10种HHV-6糖蛋白和8种HHV-6多肽与感染细胞表面结合，被人血清识别。人血清免疫沉淀的糖蛋白分子量与单克隆抗体免疫沉淀的糖蛋白分子量相似。HHY-6蛋白条带的标记强度随IF滴度的增加而增加，其中以HHV-6糖肽的作用最为显著。与特异性HHV-6多肽无反应性是特定患者组的特征。这些结果表明HHV-6糖蛋白是人抗体应答的良好靶点。
Proteins of human herpesvirus 6 (HHV-6) eliciting human antibody responses were examined in serum from healthy adults and patients with AIDS, chronic fatigue syndrome, Hodgkin's disease, and Sjogren's syndrome. HHV-6IgG antibody titers measured byimmunofluorescence(IF) ranged from 1:10 to 1:1280. LysatesofHHV-6-infected and uninfected cells labeled with pSS]methionine, [3H]glucosamine, and uSIwereimmunoprecipitated with sera and analyzed electophoretically. Sera with IF titers ⩾1:20 immunoprecipitated >20 [35S]methionine-labeled HHV-6 polypeptides of ∼26–180 kDa. At least 10 HHV-6 glycoproteins and 8 HHV-6 polypeptides associated with the surfaces of infected cells were recognized by human sera. The approximate molecular masses of glycoproteins immunoprecipitated by human sera weresimilar to those immunoprecipitated by monoclonal antibodies. The labeling intensity of HHY-6 protein bands increased with increasingIF titer, and the effectwasmost prominent for HHV-6 glycopolypeptides. No reactivities with specificHHV-6polypeptide(s) were characteristic of a given patient group. These findings suggest that HHV-6glycoproteinsare good targets for human antibody responses.