Intercellular Junctions In Fanft‐Induced Carcinomas Of Rat Urinary Bladder In Tissue Culture: In Situ Thin‐Section, Freeze‐Fracture, And Scanning Electron Microscopy Studies
Intercellular Junctions In Fanft‐Induced Carcinomas Of Rat Urinary Bladder In Tissue Culture: In Situ Thin‐Section, Freeze‐Fracture, And Scanning Electron Microscopy Studies
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组织培养中 Fanft 诱导的大鼠膀胱癌的细胞间连接：原位薄切片、冷冻断裂和扫描电子显微镜研究
DOI：
10.1111/j.1365-2818.1979.tb00178.x
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发表时间：
1979
影响因子：
2
通讯作者：
R. Weinstein
中科院分区：
文献类型：
--
作者：
B. Pauli;K. Kuettner;R. Weinstein
本文描述了一套用于对源自非侵袭性和侵袭性致癌物诱导的大鼠膀胱癌的组织培养肿瘤细胞进行比较光学显微镜和电子显微镜研究的简单方法。细胞在热那克斯塑料盖玻片上生长并原位固定。然后用剪刀将每个塑料盖玻片分成四部分：第一部分用于光学显微镜处理，第二部分用于超薄切片电子显微镜，第三部分用于冷冻断裂电子显微镜，第四部分用于扫描电子显微镜。在一些实验中，首先通过光学显微镜检查的部分培养物随后被制备用于电子显微镜观察。通过这种方式，培养条件保持恒定，并且可以通过几种制备技术对结构特征（即细胞间连接）进行比较。
This paper describes a set of simple methods for comparative light and electron microscopy studies on tissue cultured tumour cells derived from both noninvasive and invasive carcinogen‐induced rat urinary bladder carcinomas. Cells are grown on Thermanox plastic coverslips and fixed in situ. Each plastic coverslip is then divided with scissors into four parts: the first is processed for light microscopy, the second for thin‐section electron microscopy, the third for freeze‐fracture electron microscopy, and the fourth for scanning electron microscopy. In some experiments, portions of the culture which have first been examined by light microscopy are subsequently prepared for electron microscopy. In this way, the culture conditions are kept constant and a comparison of structural features (i.e. intercellular junctions) by several preparative techniques is possible.