Multifunctional oval-shaped gold-nanoparticle-based selective detection of breast cancer cells using simple colorimetric and highly sensitive two-photon scattering assay.
Multifunctional oval-shaped gold-nanoparticle-based selective detection of breast cancer cells using simple colorimetric and highly sensitive two-photon scattering assay.
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使用简单的比色和高灵敏度双光子散射测定法，基于多功能椭圆形金纳米粒子选择性检测乳腺癌细胞。
DOI：
10.1021/nn901742q
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发表时间：
2010-03-23
期刊：
影响因子：
17.1
通讯作者：
Ray, Paresh Chandra
中科院分区：
文献类型：
--
作者：
Lu, Wentong;Arumugam, Ranjini;Senapati, Dulal;Singh, Anant K.;Arbneshi, Tahir;Khan, Sadia Afrin;Yu, Hongtao;Ray, Paresh Chandra
乳腺癌是女性最常见的癌症，也是当今女性癌症死亡的第二大原因。癌症等疾病的有效和最终成功治疗的关键是早期和准确的诊断。在这种需要的推动下，我们首次报道了一种简单的比色和高灵敏的双光子散射分析方法，用于高选择性和高灵敏度地检测乳腺癌SK-BR-3细胞系，使用多功能(抗HER2/c-erb-2单抗和S6 RNA适体偶联的单抗)椭圆形金纳米颗粒纳米结合物，在100细胞/毫升水平上高选择性和灵敏地检测乳腺癌SK-BR-3细胞系。当多功能椭圆形金纳米粒子与乳腺癌SK-BR-3细胞株混合时，发生了明显的颜色变化，双光子散射强度增加了约13倍。用HaCaT非癌细胞系和MDA-MB-231乳腺癌细胞系的实验数据清楚地表明，我们的方法对SK-BR-3高度敏感，并能够与其他低水平表达HER-2的乳腺癌细胞系区分开来。讨论了选择性和分析响应变化的机理。我们在这里报道的实验结果为通过监测多功能金纳米系统的比色变化和测量TPS强度而快速、容易和可靠地诊断癌细胞开辟了新的可能性。
Breast cancer is the most common cancer among women and it is the second leading cause of cancer deaths in women today. The key to the effective and ultimately successful treatment of diseases such as cancer is an early and accurate diagnosis. Driven by the need, in this article, we report for the first time a simple colorimetric and highly sensitive two-photon scattering assay for highly selective and sensitive detection of breast cancer SK-BR-3 cell lines in 100-cells/ml level using multifunctional (monoclonal anti-HER2/c-erb-2 antibody and S6 RNA aptamers conjugated) oval shape gold nanoparticle based nanoconjugate. When multifunctional oval shape gold nanoparticles were mixed with breast cancer SK-BR-3 cell line, a distinct color change occurs and two-photon scattering intensity increases by about 13 times. Experimental data with HaCaT non-cancerous cell line, as well as with MDA-MB-231 breast cancer cell line clearly demonstrated that our assay was highly sensitive to SK-BR-3 and it was able to distinguish from other breast cancer cell line which expresses low levels of HER-2. The mechanism of selectivity and assay’s response change, have been discussed. Our experimental results reported here open up a new possibility of rapid, easy and reliable diagnosis of cancer cell lines by monitoring the colorimetric change and measuring TPS intensity from multifunctional gold nanosystems.
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影响因子：
15
作者：
Agasti SS;Chompoosor A;You CC;Ghosh P;Kim CK;Rotello VM
通讯作者：
Rotello VM
影响因子：
17.1
作者：
Bhirde, Ashwin A.;Patel, Vyomesh;Gavard, Julie;Zhang, Guofeng;Sousa, Alioscka A.;Masedunskas, Andrius;Leapman, Richard D.;Weigert, Roberto;Gutkind, J. Silvio;Rusling, James F.
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Rusling, James F.
影响因子：
3.3
作者：
Das, PK
通讯作者：
Das, PK
影响因子：
2
作者：
Huang, Yuan-Ko;Chen, Chao-Chun;Lee, Chiang
通讯作者：
Lee, Chiang
影响因子：
56.9
作者：
Fang, Ying;Seong, Nak-Hyun;Dlott, Dana D.
通讯作者：
Dlott, Dana D.