Near-isogenic lines developed for a major QTL on chromosome arm 4HL conferring Fusarium crown rot resistance in barley
Near-isogenic lines developed for a major QTL on chromosome arm 4HL conferring Fusarium crown rot resistance in barley
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DOI：
10.1007/s10681-015-1623-9
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发表时间：
2016-06-01
期刊：
影响因子：
1.9
通讯作者：
Liu, Chunji
中科院分区：
文献类型：
--
作者：
Habib, Ahsan;Shabala, Sergey;Liu, Chunji
利用大麦抗镰刀菌冠腐病（FCR）主效数量性状基因座构建了10对近等基因系。该基因座位于染色体4 H的长臂上，并且抗性等位基因的存在使FCR严重程度降低了32.8%至63.4%，在这些近等基因系中平均为43.9%。组织学和定量PCR分析都证实，镰刀菌感染和疾病发展的速度慢得多，在抗性菌株与感病菌株相比。这些近等基因对中的每一个的等值线之间的FCR严重性的对比表型将有利于赋予对这种疾病的抗性的基因的克隆和功能分析的努力。
Ten pairs of near-isogenic lines (NILs) were developed for a major quantitative trait locus conferring Fusarium crown rot (FCR) resistance in barley. The locus locates on the long arm of chromosome 4H and the presence of the resistance allele reduced FCR severity by between 32.8 and 63.4 % with an average of 43.9 % across these NILs. Histological and quantitative PCR analyses all confirmed that the rates of Fusarium infection and disease development were much slower in the resistant isolines compared with those in the susceptible isolines. The contrasting phenotypes in FCR severity between the isolines for each of these NIL pairs would facilitate efforts in cloning and functional analyses of genes conferring resistance to this disease.