Plasmodium chabaudi chabaudi infection in mice induces strong B cell responses and striking but temporary changes in splenic cell distribution
Plasmodium chabaudi chabaudi infection in mice induces strong B cell responses and striking but temporary changes in splenic cell distribution
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DOI：
10.4049/jimmunol.171.1.317
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Langhorne, J
中科院分区：
文献类型：
--
作者：
Achtman, AH;Khan, M;Langhorne, J
B细胞和抗体在控制疟疾的红细胞期中起关键作用。然而，人们对感染过程中不道德反应的发展方式知之甚少。我们发现，疟原虫chabaudi chabaudi的原因主要是，但暂时的变化，白细胞在脾脏中的分布。尽管有这些变化，对感染的有序反应仍在发展，包括浆母细胞的滤泡外生长和生殖中心的形成。在反应的早期，T区和滤泡中的淋巴细胞变得间隔很大，并且这些隔室的边缘变得模糊。这种效应在寄生虫血症的峰值附近最大。衰老中心在第8天明显，在第20天达到峰值，并持续到第60天。从第4天起，可观察到浆母细胞的滤泡外病灶，并启动非常强的浆细胞反应。最初，浆细胞具有常规的红髓分布，但到第10天，它们非常规地位于白色髓的动脉周围区域。在这个区域中，它们形成簇，占据通常由T细胞填充的区域的一部分。B细胞在感染高峰后至少30天内不存在于边缘区，尽管流式细胞术显示它们在整个感染过程中持续存在于脾脏中。相对正常的脾脏结构在感染后60天恢复。这些结果表明，脾细胞分布的变化与寄生虫的存在有关，似乎不会干扰不道德反应的发展。
B cells and Abs play a key role in controlling the erythrocytic stage of malaria. However, little is known about the way the Immoral response develops during infection. We show that Plasmodium chabaudi chabaudi causes major, but temporary changes in the distribution of leukocytes in the spleen. Despite these changes, an ordered response to infection develops, which includes vigorous extrafollicular growth of plasmablasts and germinal center formation. Early in the response, the lymphocytes in the T zone and follicles become widely spaced, and the edges of these compartments blur. This effect is maximal around the peak of parasitemia. Germinal centers are apparent by day 8, peak at day 20, and persist through day 60. Extrafollicular foci of plasmablasts are visible from day 4 and initiate a very strong plasma cell response. Initially, the plasma cells have a conventional red pulp distribution, but by day 10 they are unconventionally sited in the periarteriolar region of the white pulp. In this region they form clusters occupying part of the area normally filled by T cells. B cells are absent from the marginal zone for at least 30 days after the peak of infection, although flow cytometry shows their continued presence in the spleen throughout infection. Relatively normal splenic architecture is regained by day 60 of infection. These results show that the changes in splenic cell distribution are linked to the presence of parasites and do not seem to interfere with the development of the Immoral response.