Measuring light-induced fungal ethylene production enables non-destructive diagnosis of disease occurrence in harvested fruits
Measuring light-induced fungal ethylene production enables non-destructive diagnosis of disease occurrence in harvested fruits
复制标题
测量光诱导真菌乙烯的产生可以对收获的水果中的疾病发生进行无损诊断
DOI：
10.1016/j.foodchem.2019.125827
复制
发表时间：
2020-04-25
期刊：
影响因子：
8.8
通讯作者：
Xu, Ling
中科院分区：
文献类型：
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作者：
Guo, Han;Liu, Anran;Xu, Ling
病原真菌给水果造成巨大损失，而乙烯（ET）与水果作物的疾病发展有关。在这项研究中，光可能通过光化学反应产生的自由基，增强了几种真菌病原体的 ET 产生。实时气体分析显示，当真菌培养物从暗培养到亮培养 (DTL) 时，ET 产量急剧增加。同样，即使使用植物 ET 合成抑制剂吡嗪酰胺，光也会加速受灰霉病感染的拟南芥植物中 ET 的产生，这表明真菌在宿主入侵期间负责 ET 的产生。此外，在受灰霉病感染的番茄和葡萄中观察到 DTL 转变后 ET 产量急剧增加，但在健康或受伤的水果中则没有。总而言之，这些发现表明 DTL 诱导的 ET 对真菌感染的植物材料具有特异性，因此代表了收获果实的无损疾病诊断的候选标记。
Pathogenic fungi cause enormous losses to fruits, and ethylene (ET) is associated with disease development in fruit crops. In this study, ET production of several fungal pathogens was enhanced by light, probably through the free radicals produced by photochemical reactions. Real-time gas analysis showed a sharp increase in ET production when fungal cultures were moved from dark-to-light (DTL). Similarly, light accelerated ET production in the Botrytis cinerea-infected Arabidopsis thaliana plants even when pyrazinamide, the inhibitor for plant ET synthesis, was applied, suggesting that the fungus is responsible for ET production during host invasion. Furthermore, a sharp increase in ET production after DTL transition was observed in B. cinerea-infected tomatoes and grapes, but not in healthy or physically wounded fruits. Taken together, these findings indicate that the DTL-induced ET is specific to the plant materials with fungal infection, and thus represents a candidate marker for non-destructive disease diagnosis of harvested fruits.