CLONING AND EXPRESSION OF THE HUMAN ERYTHROPOIETIN GENE
CLONING AND EXPRESSION OF THE HUMAN ERYTHROPOIETIN GENE
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DOI：
10.1073/pnas.82.22.7580
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
GOLDWASSER, E
中科院分区：
文献类型：
--
作者：
LIN, FK;SUGGS, S;GOLDWASSER, E
用混合20-聚体和17-聚体寡核苷酸探针从噬菌体文库中分离出人促红细胞生成素基因。该基因的整个编码区包含在5.4千碱基的HindIII-BamHI片段中。该基因包含4个中间序列（1562个碱基对）和5个外显子（582个碱基对）。它编码一个27个氨基酸的信号肽和一个166个氨基酸的成熟蛋白，计算Mr为18,399。将促红细胞生成素基因导入中国仓鼠卵巢细胞，产生体外和体内均具有生物活性的促红细胞生成素。
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.