FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato.
FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato.
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FIS1编码一种调节番茄果实硬度的GA2-氧化酶。
DOI：
10.1038/s41467-020-19705-w
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发表时间：
2020-11-17
影响因子：
16.6
通讯作者：
Cui X
中科院分区：
文献类型：
--
作者：
Li R;Sun S;Wang H;Wang K;Yu H;Zhou Z;Xin P;Chu J;Zhao T;Wang H;Li J;Cui X
果实硬度是番茄育种的目标性状，因为它有利于运输和储存。然而，它也是一个复杂的性状，揭示控制果实硬度的分子遗传机制已被证明具有挑战性。在这里，我们报告的qFIRM皮肤1（qFIS 1），一个主要的数量性状基因座，部分决定了栽培和野生番茄品种之间的抗压性差异的图位克隆和功能特性。FIS 1编码一种GA 2-氧化酶，其突变导致生物活性赤霉素含量增加，角质和蜡生物合成增强，果实硬度和货架期增加。重要的是，FIS 1对果实重量或味道没有不利影响，使其成为育种者的理想目标。我们的研究表明，FIS 1介导赤霉素催化并调节果实硬度，这为番茄育种提供了一个潜在的策略，以生产更坚实的果实。果实硬度是育种者的重要指标，也是许多水果货架期的关键决定因素。在这里，作者表明，突变番茄FIS 1，一种GA 2氧化酶，增加了生物活性赤霉素的浓度，增强了角质和蜡的生物合成，并增加了果实的硬度。
Fruit firmness is a target trait in tomato breeding because it facilitates transportation and storage. However, it is also a complex trait and uncovering the molecular genetic mechanisms controlling fruit firmness has proven challenging. Here, we report the map-based cloning and functional characterization of qFIRM SKIN 1 (qFIS1), a major quantitative trait locus that partially determines the difference in compression resistance between cultivated and wild tomato accessions. FIS1 encodes a GA2-oxidase, and its mutation leads to increased bioactive gibberellin content, enhanced cutin and wax biosynthesis, and increased fruit firmness and shelf life. Importantly, FIS1 has no unfavorable effect on fruit weight or taste, making it an ideal target for breeders. Our study demonstrates that FIS1 mediates gibberellin catabolism and regulates fruit firmness, and it offers a potential strategy for tomato breeders to produce firmer fruit. Fruit firmness is an important target for breeders and a key determinant of shelf life for many fruits. Here the authors show that mutating tomato FIS1, a GA2-oxidase, increases the concentration of bioactive gibberellins, enhances cutin and wax biosynthesis and increases fruit firmness.