Phosphorescence monitoring of hypoxic microenvironment in solid-tumors to evaluate chemotherapeutic effects using the hypoxia-sensitive iridium (III) coordination compound.
Phosphorescence monitoring of hypoxic microenvironment in solid-tumors to evaluate chemotherapeutic effects using the hypoxia-sensitive iridium (III) coordination compound.
复制标题
使用缺氧敏感铱 (III) 配位化合物对实体瘤中的缺氧微环境进行磷光监测以评估化疗效果
DOI：
10.1371/journal.pone.0121293
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wu D
中科院分区：
文献类型：
--
作者：
Zeng Y;Liu Y;Shang J;Ma J;Wang R;Deng L;Guo Y;Zhong F;Bai M;Zhang S;Wu D
Objectives To utilize phosphorescence to monitor hypoxic microenvironment in solid-tumors and investigate cancer chemotherapeutic effects in vivo. Methods A hypoxia-sensitive probe named BTP was used to monitor hypoxic microenvironment in solid-tumors. The low-dose metronomic treatment with cisplatin was used in anti-angiogenetic chemotherapeutic programs. The phosphorescence properties of BTP were detected by a spectrofluorometer. BTP cytotoxicity utilized cell necrosis and apoptosis, which were evaluated by trypan blue dye exclusion and Hoechst33342 plus propidium iodide assays. Tumor-bearing mouse models of colon adenocarcinoma were used for tumor imaging in vivo. Monitoring of the hypoxic microenvironment in tumors was performed with a Maestro 2 fluorescence imaging system. Tumor tissues in each group were harvested regularly and treated with pathological hematoxylin and eosin and immunohistochemical staining to confirm imaging results. Results BTP did not feature obvious cytotoxicity for cells, and tumor growth in low-dose metronomic cisplatin treated mice was significantly inhibited by chemotherapy. Hypoxic levels significantly increased due to cisplatin, as proven by the expression level of related proteins. Phosphorescence intensity in the tumors of mice in the cisplatin group was stronger and showed higher contrast than that in tumors of saline treated mice. Conclusions We develop a useful phosphorescence method to evaluate the chemotherapeutic effects of cisplatin. The proposed method shows potential as a phosphorescence imaging approach for evaluating chemotherapeutic effects in vivo, especially anti-angiogenesis.
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影响因子：
3.7
作者：
Adamski J;Price A;Dive C;Makin G
通讯作者：
Makin G
影响因子：
8
作者：
Dmitriev, Ruslan I.;Papkovsky, Dmitri B.
通讯作者：
Papkovsky, Dmitri B.
DOI：
10.1111/j.1365-2613.2009.00684.x
发表时间：
2010-02-01
影响因子：
3
作者：
Shen, Fang-Zhen;Wang, Jing;Wang, Yan-Tao
通讯作者：
Wang, Yan-Tao
DOI：
10.1007/978-3-642-27994-2_15
发表时间：
2013-01-01
期刊：
Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer
影响因子：
--
作者：
Hoigebazar, Lathika;Jeong, Jae Min
通讯作者：
Jeong, Jae Min
影响因子：
10.6
作者：
Segard, Tatiana;Robins, Peter D.;Francis, Roslyn J.
通讯作者：
Francis, Roslyn J.