Effects of temperature on series elasticity and contractile element motion in heart muscle.
Effects of temperature on series elasticity and contractile element motion in heart muscle.
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温度对心肌系列弹性和收缩元件运动的影响。
DOI：
10.1152/ajplegacy.1969.217.4.1030
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
E. Sonnenblick
中科院分区：
文献类型：
--
作者：
L. Yeatman;W. Parmley;E. Sonnenblick
材料与方法雄性Wistar大鼠1.5- 2.0kg，戊巴比妥钠50 mg/kg腹腔注射麻醉。迅速取出右心室乳头肌，并置于含有Krebs-Ringer溶液的10-ml肌肉浴中，该溶液在29 ℃下用95%O2和5%CO2充气。以1 Z/min的速率刺激肌肉，脉冲持续时间为5-7毫秒，电压高于阈值10%。肌肉在下方固定在弹簧加载夹中，弹簧加载夹是张力传感器的刚性延伸，并且通过线夹将上方连接到等张杠杆系统（9）的末端。初始肌肉长度通过小的预加载设定，并通过千分尺挡块保持恒定。在浴内稳定至少1小时后，获得肌肉的被动和主动长度-张力曲线，并从主动曲线的上升部分选择三个预载荷。在29 ℃下，在这些预载荷下获得GE力-速度和SE载荷-延伸曲线。然后将温度降低到24 ℃，并在该温度下以及再次在29、34和37 ℃下获得相同预载荷的SE和力-速度曲线。此外，四块肌肉在22 ℃下进行了研究，一块肌肉的温度首先升高到37 ℃，然后降低到24 ℃。在24 ℃和34 ℃下测定了三种肌肉的长-张力曲线。
MATERIALS AND METHODSCats (1.5-2.0 kg) were anesthetized by intraperitoneal injection of 50 mg/kg of sodium pentobarbital. Right ventricular papillary muscles were rapidly removed and placed in a IO-ml muscle bath containing Krebs-Ringer solution aerated with 95% 02 and 5% CO2 at 29 C. The muscles were stimulated at a rate of lZ/min, with a pulse duration of 5-7 msec at a voltage 10% above threshold. The muscles were fixed below in a spring-loaded clip, which was a rigid extension of a tension transducer, and were attached above by a wire clip to the end of an isotonic lever system (9). Initial muscle length was set by a small preload and kept constant by a micrometer stop. After at least 1 hr of stabilization within the bath, both passive and active length-tension curves of the muscles were obtained, and three preloads selected from the rising portion of the active curve. GE force-velocity and SE load-extension curves were obtained at these preloads at 29 C. Temperature was then lowered to 24 C and the SE and force-velocity curves obtained for the same preloads at that temperature, and again at 29, 34, and 37 C. In addition, four muscles were studied at 22 C, and one studied with temperature first raised to 37 C and then lowered to 24 C. Lengthtension curves were determined at 24 C and 34 C in three muscles.