Accumulation of pathogenesis-related proteins in the apoplast of a susceptible cultivar of apple (Malus domestica cv. Elstar) after infection by Venturia inaequalis and constitutive expression of PR genes in the resistant cultivar Remo
Accumulation of pathogenesis-related proteins in the apoplast of a susceptible cultivar of apple (Malus domestica cv. Elstar) after infection by Venturia inaequalis and constitutive expression of PR genes in the resistant cultivar Remo
复制标题
DOI：
10.1023/b:ejpp.0000041552.98673.e3
复制
发表时间：
2004-08-01
影响因子：
1.8
通讯作者：
Kloppstech, K
中科院分区：
文献类型：
--
作者：
Gau, AE;Koutb, M;Kloppstech, K
苹果叶（Malus Domestica cv. Elstar）被苹果黑星病 Venturia inaequalis 的克隆分离株感染。收集这些植物的细胞间冲洗液（IWF），并通过 SDS-PAGE 和二维凝胶电泳分析苹果黑星病病原 V. inaequalis 感染期间和感染后 IWF 中蛋白质组成的变化。随后通过电子喷雾电离四极杆飞行时间质谱法对诱导蛋白进行分析，结果显示在被黑马菌感染的家蝇叶子中存在β-1,3-葡聚糖酶、几丁质酶、类索马甜蛋白和类半胱氨酸蛋白酶。这些结果通过针对其中一些蛋白质的抗体的免疫印迹得到证实。此外，在未感染的叶子中发现了一种非特异性脂质转移蛋白：在被V. inaequalis感染后的第一周内，其数量下降至不可检测的水平。家蝇 IWF 分析。 Remo 对苹果黑星病、白粉病和火疫病具有抗性，其蛋白质模式与来自品种 Elstar 的黑星弧菌感染叶子的 IWF 的蛋白质模式相当，表明抗性品种中至少有一些发病机制相关蛋白质的组成型产生。
Leaves of apple ( Malus domestica cv. Elstar) were infected with a cloned isolate of the apple scab Venturia inaequalis. The intercellular washing fluid (IWF) of these plants was collected and the variation in the composition of proteins in the IWF was analysed by SDS-PAGE and two-dimensional gel electrophoresis during and after the infection with V. inaequalis, the causal agent of apple scab. The subsequent analysis of induced proteins by electron spray ionization quadrupole time of flight mass spectroscopy revealed the presence of beta-1,3-glucanase, chitinase, thaumatin-like protein and a cysteine-like protease in M. domestica leaves infected by V. inaequalis. These results were confirmed by immunoblotting with antibodies against some of these proteins. Moreover, a non-specific lipid transfer protein was identified in uninfected leaves: the amount declined to a non-detectable level within the first week after infection by V. inaequalis. The analysis of the IWF of M. domestica cv. Remo, bearing resistances to apple scab, powdery mildew and fire blight, showed a protein pattern comparable to that of the IWF from V. inaequalis infected leaves from cultivar Elstar indicating the constitutive production at least of some of the pathogenesis-related proteins in the resistant cultivar.