PCR-based assay for detecting 1B-genes for low molecular weight glutenin subunits related to gluten quality properties in durum wheat
PCR-based assay for detecting 1B-genes for low molecular weight glutenin subunits related to gluten quality properties in durum wheat
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DOI：
10.1111/j.1439-0523.1996.tb00944.x
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发表时间：
1996-11-01
期刊：
影响因子：
2
通讯作者：
Porceddu, E
中科院分区：
文献类型：
--
作者：
DOvidio, R;Porceddu, E
建立了一种筛选与硬粒小麦品质性状相关的位于1B染色体上的低分子量谷蛋白基因的聚合酶链式反应方法。大多数具有良好技术特性的硬粒小麦品系可以根据特定的1B低分子量麦谷蛋白组分的存在来鉴定，这些组份被指定为LMW-2麦谷蛋白亚基。根据与低分子量麦谷蛋白基因对应的核苷酸序列，制备了一系列特异的寡核苷酸引物，并用于聚合酶链式反应分析。其中一对扩增产物可区分含有LMW-2麦谷蛋白亚基的硬粒小麦品系和含有其他1B-LMW等位基因变异的品系。因为所提出的聚合酶链式反应分析可以使用部分胚乳进行。为加快含LMW-2麦谷蛋白亚基的品种选育提供了一条有效途径。这部分引物在基因组图谱分析和小麦品种纯度检测中也可能非常有用。
A PCR assay has been developed for selecting low molecular weight (LMW) glutenin genes, located on chromosome 1B, and related to durum wheat quality characteristics. Most durum wheat lines possessing good technological properties can be identified on the basis of the presence of specific 1B LMW glutenin components that have been designated LMW-2 glutenin subunits. On the basis of nucleotide sequences corresponding to LMW glutenin genes, a series of specific oligonucleotide primers were prepared and used in PCR analysis. A pair of these primers gave a single amplification product which can distinguish between durum wheat lines possessing LMW-2 glutenin subunits and Lines possessing other 1B-LMW allelic variants. Because the proposed PCR analysis can be carried out using part of the endosperm. of a single seed, it represents a helpful approach for speeding up the selection of genotypes possessing LMW-2 glutenin subunits. This part of primers could also be very useful in genome mapping analysis and for testing the purity of wheat hour stock.