Second-Harmonic-Generation Microscopy Using Excitation Beam with Controlled Polarization Pattern to Determine Three-Dimensional Molecular Orientation
Second-Harmonic-Generation Microscopy Using Excitation Beam with Controlled Polarization Pattern to Determine Three-Dimensional Molecular Orientation
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DOI：
10.1143/jjap.44.l1066
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发表时间：
2005-08
影响因子：
1.5
通讯作者：
K. Yoshiki;M. Hashimoto;T. Araki
中科院分区：
文献类型：
--
作者：
K. Yoshiki;M. Hashimoto;T. Araki
我们已经开发了一种二次谐波发生（SHG）显微镜，使用一个控制偏振模式的激发光束，以检测三维分子取向。焦点处的电场通过用平行排列的向列型液晶空间光调制器修改偏振分布而被三维地控制，而没有任何机械移动部件。我们证明，从跟腱，切片，使胶原纤维平行于光轴排列，激发的径向偏振光束的SHG信号高于那些激发的线性偏振光束。从而显示了测定分子三维取向的可能性。
We have developed a second-harmonic-generation (SHG) microscope using an excitation beam with a controlled polarization pattern in order to detect three-dimensional molecular orientation. The electric field at the focus is controlled three-dimensionally by modifying the polarization distribution with a parallel-aligned nematic-liquid-crystal spatial-light-modulator without any mechanical moving parts. We demonstrated that the SHG signal from an Achilles tendon, sliced so that collagen fibers were aligned parallel to the optical axis, excited by a radially polarized beam was higher than those excited by linearly polarized beams. The possibility of determinating three-dimensional molecular orientation was thus shown.