Identification of two QTLs for resistance to Fusarium wilt race 1 in lettuce (Lactuca sativa L.)
Identification of two QTLs for resistance to Fusarium wilt race 1 in lettuce (Lactuca sativa L.)
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生菜 (Lactuca sativa L.) 抗枯萎病小种 1 的两个 QTL 的鉴定
DOI：
10.1007/s10681-020-02713-8
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发表时间：
2020
期刊：
影响因子：
1.9
通讯作者：
H. Matsumura
中科院分区：
文献类型：
--
作者：
Kousuke Seki;K. Komatsu;M. Hiraga;Keisuke Tanaka;Y. Uno;H. Matsumura
枯萎病1号生理小种由尖孢镰刀菌（Fusarium oxysporum f.莴苣的主要病害之一是热带和亚热带地区莴苣的主要病害，包括在日本的夏季条件下。抗莴苣品种有时会表现出症状，这使得育种计划将重点放在改善对1号小种的抗性表型上。利用枯萎病生理小种1进行病害测定，莴苣品种“VI 185”被归类为高抗性，这通过随后的群体分析得到验证。分析从“VI 185”（高抗性）和“ShinanoGreen”（部分抗性）之间的杂交获得的F2个体，并使用生理小种1病原体对F3群体进行感染测定。ddRAD-seq分析揭示了LG 7（qFOL7.1）和LG 8（qFOL8.1）上的两个显著QTL，其解释了1号生理小种抗性表型。此外，在两个QTL上，赋予高抗性的位点都起源于“VI 185”。我们的研究结果提供了一个基础，了解QTL的抗枯萎病生理小种1，并提供证据表明，遗传多样性是可用于提高现有的生菜品种的抗性。
Fusarium wilt race 1, caused by Fusarium oxysporum f. sp. lactucae, is one of the main diseases of lettuce in tropical and subtropical regions, including under summer conditions in Japan. Resistant lettuce cultivars have shown symptoms on occasion, which have led breeding programs to focus on the improvement of resistance phenotypes to race 1. Disease assays using Fusarium wilt race 1 were performed, and the lettuce cultivar ‘VI185’ was categorized as highly resistant, which was validated by subsequent population analyses. F2 individuals obtained from a cross between ‘VI185’ (highly resistant) and ‘ShinanoGreen’ (partially resistant) were analyzed, and an F3 population was subjected to an infection assay using the race 1 pathogen. ddRAD-seq analysis revealed two significant QTLs on LG7 (qFOL7.1) and LG8 (qFOL8.1) that accounted for the race 1 resistance phenotype. Furthermore, the loci that conferred elevated resistance originated from ‘VI185’ at both QTLs. Our results provide a foundation for understanding the QTLs for resistance to Fusarium wilt race 1 and provide evidence that genetic diversity is available for enhancing the current resistance in existing lettuce cultivars.