Analysis of 11 tetrameric STRs in wild boars for forensic purposes
Analysis of 11 tetrameric STRs in wild boars for forensic purposes
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DOI：
10.1016/j.fsigen.2010.07.001
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发表时间：
2010-10-01
影响因子：
3.1
通讯作者：
Robino, Carlo
中科院分区：
文献类型：
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作者：
Caratti, Stefano;Rossi, Luca;Robino, Carlo
动物物种的STR分析具有广泛的应用，包括法医鉴定、野生动物保护、兽医公共卫生保护和食品安全。我们测试了一种基于多重PCR的检测方法的有效性，该方法包括来自皮埃蒙特（意大利西北部）的野猪（n = 142）群体样本中的11个猪特异性四聚体STR。观察到多个偏离Hardy-Weinberg预期，主要是由于观察到的杂合性降低，表明高度近亲繁殖。估计θ值为0.046，近交系数为0.089。STR组合的鉴别力和排除概率值分别为0.999999999996和0.99989。为了测试该方法对肉类可追溯性目的的适用性，还对由肉类生产过程中常用的商业品系组成的家猪参考样品（n = 412）进行了分型。贝叶斯聚类分析进行了观察到的基因型，显示正确的亚种分配的百分比为0.974野猪和0.991猪的个体样本，从而证明了有用的多重STR分型系统的歧视目的。(C)2010爱思唯尔爱尔兰有限公司版权所有。
STR profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety. We tested the efficacy of a multiplex PCR-based assay including 11 porcine-specific tetrameric STRs in a population sample of wild boars (n = 142) originating from Piedmont (North West Italy). Multiple deviations from Hardy-Weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative of a high degree of inbreeding. A value of theta of 0.046 and an inbreeding coefficient of 0.089 were estimated. Combined power of discrimination and probability of exclusion values for the STR panel were 0.9999999999996 and 0.99989. In order to test the suitability of the method for meat traceability purposes, a domestic pig reference sample (n = 412), consisting of commercial lines commonly used in the meat production process, was also typed. A Bayesian cluster analysis carried out using the observed genotypes, showed a percentage of correct subspecies assignment of individual samples of 0.974 for wild boars and 0.991 for pigs, thus demonstrating the usefulness of the multiplex STR-typing system for discrimination purposes. (C) 2010 Elsevier Ireland Ltd. All rights reserved.